{"doi":"10.1007/s11060-015-1784-3","title":"miRNA contents of cerebrospinal fluid extracellular vesicles in glioblastoma patients","abstract":null,"journal":"Journal of Neuro-Oncology","year":2015,"id":612298,"datarank":4.834454231616932,"base_score":5.0369526024136295,"endowment":5.0369526024136295,"self_citation_contribution":0.7555428903620446,"citation_network_contribution":4.078911341254887,"self_endowment_contribution":0.7555428903620446,"citer_contribution":4.078911341254887,"corpus_percentile":null,"corpus_rank":null,"citation_count":153,"citer_count":142,"citers_with_citation_signal":129,"citers_with_endowment":129,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":346346,"name":"Valya Ramakrishnan","orcid":null,"position":1,"is_corresponding":false},{"id":240918,"name":"Ryan Kim","orcid":"0000-0003-2007-5707","position":2,"is_corresponding":false},{"id":1225829,"name":"Shirley Phillips","orcid":null,"position":3,"is_corresponding":false},{"id":1576483,"name":"Vivek Kaimal","orcid":null,"position":4,"is_corresponding":false},{"id":197284,"name":"Ying Mao","orcid":null,"position":5,"is_corresponding":false},{"id":1214927,"name":"Wei Hua","orcid":"0000-0003-3157-5715","position":6,"is_corresponding":false},{"id":435921,"name":"Isaac Yang","orcid":"0000-0002-5176-5615","position":7,"is_corresponding":false},{"id":1576484,"name":"Chia-Chun Fu","orcid":null,"position":8,"is_corresponding":false},{"id":215765,"name":"John Nolan","orcid":null,"position":9,"is_corresponding":false},{"id":254088,"name":"Ichiro Nakano","orcid":"0000-0002-0916-3207","position":10,"is_corresponding":false},{"id":1090477,"name":"Yuanfan Yang","orcid":"0000-0003-1581-451X","position":11,"is_corresponding":false},{"id":1576485,"name":"Martin Beaulieu","orcid":null,"position":12,"is_corresponding":false},{"id":95876,"name":"Bob S. Carter","orcid":"0000-0002-3586-1324","position":13,"is_corresponding":false},{"id":746435,"name":"Clark C. Chen","orcid":"0000-0001-6258-9277","position":14,"is_corresponding":false},{"id":1576477,"name":"Johnny C. Akers","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"miRNA contents of cerebrospinal fluid extracellular vesicles in glioblastoma patients","abstract":"Analysis of extracellular vesicles (EVs) derived from plasma or cerebrospinal fluid (CSF) has emerged as a promising biomarker platform for therapeutic monitoring in glioblastoma patients. However, the contents of the various subpopulations of EVs in these clinical specimens remain poorly defined. Here we characterize the relative abundance of miRNA species in EVs derived from the serum and cerebrospinal fluid of glioblastoma patients. EVs were isolated from glioblastoma cell lines as well as the plasma and CSF of glioblastoma patients. The microvesicle subpopulation was isolated by pelleting at 10,000×g for 30 min after cellular debris was cleared by a 2000×g (20 min) spin. The exosome subpopulation was isolated by pelleting the microvesicle supernatant at 120,000×g (120 min). qRT-PCR was performed to examine the distribution of miR-21, miR-103, miR-24, and miR-125. Global miRNA profiling was performed in select glioblastoma CSF samples. In plasma and cell line derived EVs, the relative abundance of miRNAs in exosome and microvesicles were highly variable. In some specimens, the majority of the miRNA species were found in exosomes while in other, they were found in microvesicles. In contrast, CSF exosomes were enriched for miRNAs relative to CSF microvesicles. In CSF, there is an average of one molecule of miRNA per 150-25,000 EVs. Most EVs derived from clinical biofluids are devoid of miRNA content. The relative distribution of miRNA species in plasma exosomes or microvesicles is unpredictable. In contrast, CSF exosomes are the major EV compartment that harbor miRNAs.","is_dataset_classified":null,"base_score":5.0369526024136295,"endowment":5.0369526024136295,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25903655","pmcid":"PMC4459648","openalex_id":"https://openalex.org/W2067120071","authors":[],"funders":[{"funder_name":"National Institutes of Health","grant_id":"UH2 TR000931-0","title":null},{"funder_name":"National Institutes of Health","grant_id":"PO1 2P30CA023100-28","title":null},{"funder_name":"International S&T Cooperation Program of China","grant_id":"2014DFA31470","title":null},{"funder_name":"Doris Duke Charitable Foundation","grant_id":"2010052","title":null},{"funder_name":"Burroughs Wellcome Fund","grant_id":"1006774.01","title":null},{"funder_name":"Sontag Foundation","grant_id":"Distinguished Scientist Award","title":null},{"funder_name":"Sidney Kimmel Foundation for Cancer Research","grant_id":"Fund","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P30 CA023100","title":null},{"funder_name":"NCATS NIH HHS","grant_id":"UH2 TR000931","title":null},{"funder_name":"NCATS NIH HHS","grant_id":"UH3 TR000931","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P01 2P30CA023100-28","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P01 CA069246","title":null},{"funder_name":"National Institutes of Health","grant_id":"2P30CA023100-28","title":"Planning and Evaluation"},{"funder_name":"National Institutes of Health","grant_id":"5UH2TR000931-02","title":"exRNA Biomarkers for Human Glioma"}],"total_grants":14,"fwci":6.2008,"citation_percentile":0.97285384,"influential_citations":0,"citation_trend":[{"year":2015,"count":2},{"year":2016,"count":10},{"year":2017,"count":22},{"year":2018,"count":11},{"year":2019,"count":17},{"year":2020,"count":17},{"year":2021,"count":17},{"year":2022,"count":14},{"year":2023,"count":12},{"year":2024,"count":18},{"year":2025,"count":5},{"year":2026,"count":8}],"oa_status":"green","license":"Springer TDM","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4459648","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4459648","host_type":"repository"},{"url":"http://link.springer.com/content/pdf/10.1007/s11060-015-1784-3.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s11060-015-1784-3/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s11060-015-1784-3","host_type":"publisher"},{"url":"https://doi.org/10.1007/s11060-015-1784-3","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25903655","host_type":"repository"},{"url":"https://escholarship.org/uc/item/2z15570r","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc4459648?pdf=render","host_type":""},{"url":"https://dx.doi.org/10.1007/s11060-015-1784-3","host_type":""},{"url":"https://escholarship.org/content/qt2z15570r/qt2z15570r.pdf","host_type":""},{"url":"https://doi.org/https://doi.org/10.1007/s11060-015-1784-3","host_type":""}],"fields_of_study":["Extracellular vesicles in disease","MicroRNA in disease regulation","Circular RNAs in diseases","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Extracellular Vesicles","Glioblastoma","Humans","RNA, Messenger","Tumor Cells, Cultured","Biomarkers","Reverse Transcriptase Polymerase Chain Reaction","Oligonucleotide Array Sequence Analysis","Gene Expression Profiling","MicroRNAs","Real-Time Polymerase Chain Reaction"],"keywords":["Extracellular vesicles","Cerebrospinal fluid","Glioblastoma","Microvesicles","Extracellular fluid","microRNA","Extracellular","Extracellular vesicle","Chemistry","Pathology","Medicine","Cancer research","Biology","Cell biology","Biochemistry","Gene","Messenger","Exosomes","Brain Disorders (rcdc)","Tumor Cells, Cultured","2.1 Biological and endogenous factors","32 Biomedical and Clinical Sciences (for-2020)","Neurosciences (rcdc)","Cancer","Oligonucleotide Array Sequence Analysis","Cancer (rcdc)","Brain Cancer (rcdc)","Humans (mesh)","Cultured","Reverse Transcriptase Polymerase Chain Reaction","1112 Oncology and Carcinogenesis (for)","3209 Neurosciences (for-2020)","Tumor Cells","Biomarkers (mesh)","4.2 Evaluation of markers and technologies (hrcs-rac)","Glioblastoma (mesh)","Cultured (mesh)","Biotechnology","4.2 Evaluation of markers and technologies","Oncology and Carcinogenesis","610","Biotechnology (rcdc)","Real-Time Polymerase Chain Reaction","Rare Diseases (rcdc)","MicroRNAs (mesh)","Oligonucleotide Array Sequence Analysis (mesh)","Rare Diseases","Biofluids","Genetics","3211 Oncology and carcinogenesis (for-2020)","Humans","Oncology & Carcinogenesis","RNA, Messenger","Reverse Transcriptase Polymerase Chain Reaction (mesh)","Messenger (mesh)","Biomedical and Clinical Sciences","Genetics (rcdc)","Gene Expression Profiling","Neurosciences","2.1 Biological and endogenous factors (hrcs-rac)","Gene Expression Profiling (mesh)","1109 Neurosciences (for)","Real-Time Polymerase Chain Reaction (mesh)","Brain Disorders","Brain Cancer","MicroRNAs","Oncology & Carcinogenesis (science-metrix)","Extracellular Vesicles (mesh)","RNA","Biomarkers"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T02:45:53.157851Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}