{"doi":"10.1007/s11033-020-05783-y","title":"Advanced glycation end products (AGEs) synergistically potentiated the proinflammatory action of lipopolysaccharide (LPS) and high mobility group box-1 (HMGB1) through their direct interactions","abstract":null,"journal":"Molecular Biology Reports","year":2020,"id":606858,"datarank":0.3958585994422889,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.0,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1558061,"name":"Takao Toyomura","orcid":null,"position":1,"is_corresponding":false},{"id":1558062,"name":"Mayuko Tomiyama","orcid":null,"position":2,"is_corresponding":false},{"id":1558063,"name":"Hidenori Wake","orcid":null,"position":3,"is_corresponding":false},{"id":1558064,"name":"Keyue Liu","orcid":null,"position":4,"is_corresponding":false},{"id":1558065,"name":"Kiyoshi Teshigawara","orcid":null,"position":5,"is_corresponding":false},{"id":254791,"name":"Hideo Takahashi","orcid":"0000-0003-3135-9457","position":6,"is_corresponding":false},{"id":790976,"name":"Masahiro Nishibori","orcid":"0000-0002-5565-6360","position":7,"is_corresponding":false},{"id":1558066,"name":"Shuji Mori","orcid":"0000-0002-2826-1937","position":8,"is_corresponding":false},{"id":121289,"name":"Masahiro Watanabe","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Advanced glycation end products (AGEs) synergistically potentiated the proinflammatory action of lipopolysaccharide (LPS) and high mobility group box-1 (HMGB1) through their direct interactions","abstract":"Previously, we found that advanced glycation endproducts (AGEs) directly interact with tumor necrosis factor (TNF)-like weak inducer of apoptosis, a cytokine that controls inflammation, and that this interaction inhibited its action. This finding raised the novel possibility that AGEs alter the function of other cytokines through direct interaction. To investigate this possibility, we performed comprehensive screening for candidates that interacted with AGEs using protein array analysis. The array analysis revealed that high mobility group box-1 (HMGB1) had a markedly high affinity for AGEs. HMGB1 is a representative proinflammatory damage-associated molecular pattern molecule, and is reported to interact with lipopolysaccharide (LPS) directly to exert its inflammatory function. When LPS, HMGB1, and AGEs were mixed, the mobility of HMGB1 had shifted significantly in native PAGE, suggesting that these three molecules formed a triplet complex. The addition of AGEs to the LPS-HMGB1 mixture synergistically potentiated LPS-HMGB1-stimulated TNF-α mRNA expression in macrophage-like RAW264.7 cells. In addition, using receptor knockout clones, the increased proinflammatory response by LPS-HMGB1-AGEs complex was demonstrated to be mediated via Toll-like receptor 4 and receptor for AGEs. Taken together, this study suggested that AGEs carry out their pathophysiological roles by potentiating the LPS-HMGB1-stimulated proinflammatory response through direct interactions.","is_dataset_classified":null,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32885364","pmcid":null,"openalex_id":"https://openalex.org/W3083272915","authors":[],"funders":[{"funder_name":"Japan Society for the Promotion of Science","grant_id":"18K06807","title":null},{"funder_name":"Japan Society for the Promotion of Science","grant_id":"18K14969","title":null},{"funder_name":"the Wesco Scientific Promotion Foundation","grant_id":"","title":null}],"total_grants":3,"fwci":1.1175,"citation_percentile":0.75330997,"influential_citations":0,"citation_trend":[{"year":2022,"count":3},{"year":2023,"count":6},{"year":2025,"count":3}],"oa_status":"closed","license":"https://www.springer.com/tdm","oa_locations":[{"url":"https://link.springer.com/content/pdf/10.1007/s11033-020-05783-y.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1007/s11033-020-05783-y/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1007/s11033-020-05783-y","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/32885364","host_type":"repository"}],"fields_of_study":["Advanced Glycation End Products research","Alcohol Consumption and Health Effects","Animals","Glycation End Products, Advanced","HMGB1 Protein","Inflammation","Lipopolysaccharides","Mice","RAW 264.7 Cells"],"mesh_terms":["RAW 264.7 Cells","Animals","Inflammation","Lipopolysaccharides","Glycation End Products, Advanced","HMGB1 Protein","Mice"],"keywords":["HMGB1","Proinflammatory cytokine","Glycation","Rage (emotion)","Lipopolysaccharide","Tumor necrosis factor alpha","Inflammation","Chemistry","Receptor","Macrophage","Cytokine","Cell biology","Immunology","Biology","Biochemistry","In vitro","Neuroscience","Toll-like receptor","advanced glycation end products","Receptor For Advanced Glycation End Products","High Mobility Group Box-1"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T05:18:25.867303Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}