{"doi":"10.1007/s11033-018-4206-8","title":"PCR-based genotyping of SNP markers in sheep","abstract":null,"journal":"Molecular Biology Reports","year":2018,"id":685896,"datarank":0.38474240361923057,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.0,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1791983,"name":"Giovanna Gabrielle Cruvinel","orcid":"0000-0002-6079-9235","position":1,"is_corresponding":false},{"id":1791985,"name":"Caroline Valério Moraes","orcid":"0000-0001-9467-2294","position":2,"is_corresponding":false},{"id":1791987,"name":"Flavia Aline Bressani","orcid":"0000-0002-3327-357X","position":3,"is_corresponding":false},{"id":1791988,"name":"Wilson Malagó Junior","orcid":"0000-0002-5802-4078","position":4,"is_corresponding":false},{"id":1791989,"name":"Magda Vieira Benavides","orcid":"0000-0002-0219-3163","position":5,"is_corresponding":false},{"id":1791990,"name":"Ana Carolina Souza de Chagas","orcid":"0000-0003-3939-0088","position":6,"is_corresponding":false},{"id":1791980,"name":"Simone Cristina Méo Niciura","orcid":"0000-0003-0046-0050","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"PCR-based genotyping of SNP markers in sheep","abstract":"Single nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl<sub>2</sub> and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays.","is_dataset_classified":null,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29869739","pmcid":null,"openalex_id":"https://openalex.org/W2805332106","authors":[],"funders":[{"funder_name":"Fundação de Amparo à Pesquisa do Estado de São Paulo","grant_id":"2017/01626-1","title":null},{"funder_name":"Conselho Nacional de Desenvolvimento Científico e Tecnológico","grant_id":"154225/2017-7","title":null},{"funder_name":"Coordenação de Aperfeiçoamento de Pessoal de Nível Superior","grant_id":"","title":null}],"total_grants":3,"fwci":0.4814,"citation_percentile":0.6925004,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2021,"count":2},{"year":2022,"count":3},{"year":2023,"count":2},{"year":2024,"count":3},{"year":2026,"count":1}],"oa_status":"green","license":"other-oa","oa_locations":[{"url":"http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094070","host_type":"repository"},{"url":"http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094070","host_type":"repository"},{"url":"http://link.springer.com/article/10.1007/s11033-018-4206-8/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s11033-018-4206-8.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1007/s11033-018-4206-8","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29869739","host_type":"repository"},{"url":"http://americanae.aecid.es/americanae/es/registros/registro.do?tipoRegistro=MTD&idBib=2116495","host_type":"repository"}],"fields_of_study":["Genetic and phenotypic traits in livestock","Helminth infection and control","Animal Nutrition and Physiology","Animals","DNA","DNA Primers","Genome-Wide Association Study","Genotype","Genotyping Techniques","Phenotype","Polymerase Chain Reaction","Polymorphism, Single Nucleotide","Sheep"],"mesh_terms":["Animals","DNA","Genotype","Phenotype","Sheep","Polymerase Chain Reaction","DNA Primers","Polymorphism, Single Nucleotide","Genome-Wide Association Study","Genotyping Techniques"],"keywords":["Genotyping","Molecular Inversion Probe","SNP genotyping","Biology","Single-nucleotide polymorphism","Polymerase chain reaction","Genetics","Multiplex polymerase chain reaction","Primer (cosmetics)","Genotype","Variants of PCR","Genome-wide association study","SNP","Gene","Chemistry","PCR-RFLP","Molecular markers","Ovine","Tetra-primer Arms-pcr"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T17:58:31.287599Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}