{"doi":"10.1007/s10456-024-09946-4","title":"The influences of ApoE isoforms on endothelial adherens junctions and actin cytoskeleton responding to mCRP","abstract":null,"journal":"Angiogenesis","year":2024,"id":603505,"datarank":0.3453877639491069,"base_score":2.302585092994046,"endowment":2.302585092994046,"self_citation_contribution":0.3453877639491069,"citation_network_contribution":0.0,"self_endowment_contribution":0.3453877639491069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":9,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":88673,"name":"Weiwei Lin","orcid":null,"position":1,"is_corresponding":false},{"id":494628,"name":"Qini Gan","orcid":null,"position":2,"is_corresponding":false},{"id":863921,"name":"Maohua Lei","orcid":null,"position":3,"is_corresponding":false},{"id":353321,"name":"Bin Gong","orcid":"0000-0001-9820-4762","position":4,"is_corresponding":false},{"id":1254585,"name":"Chao Zhang","orcid":"0009-0007-8631-2476","position":5,"is_corresponding":false},{"id":1178927,"name":"Jessica Salles Henrique","orcid":null,"position":6,"is_corresponding":false},{"id":776919,"name":"Jingyan Han","orcid":"0000-0001-9893-2575","position":7,"is_corresponding":false},{"id":234919,"name":"Hua Tian","orcid":"0000-0002-3598-0219","position":8,"is_corresponding":false},{"id":857481,"name":"Qiushan Tao","orcid":"0000-0002-1852-3681","position":9,"is_corresponding":false},{"id":1026855,"name":"Lawrence A. Potempa","orcid":"0000-0002-9267-1041","position":10,"is_corresponding":false},{"id":271281,"name":"Thor D. Stein","orcid":"0000-0001-6954-4477","position":11,"is_corresponding":false},{"id":253656,"name":"Andrew Emili","orcid":"0000-0001-8995-246X","position":12,"is_corresponding":false},{"id":493568,"name":"Wei Qiao Qiu","orcid":"0000-0002-2082-2410","position":13,"is_corresponding":false},{"id":493567,"name":"Zhengrong Zhang","orcid":"0000-0002-0608-8520","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The influences of ApoE isoforms on endothelial adherens junctions and actin cytoskeleton responding to mCRP","abstract":"Apolipoprotein E4 (ApoE4) plays an important role responding to monomeric C-reactive protein (mCRP) via binding to CD31 leading to cerebrovascular damage and Alzheimer's disease (AD). Using phosphor-proteomic profiling, we found altered cytoskeleton proteins in the microvasculature of AD brains, including increased levels of hyperphosphorylated tau (pTau) and the actin-related protein, LIMA1. To address the hypothesis that cytoskeletal changes serve as early pathological signatures linked with CD31 in brain endothelia in ApoE4 carriers, ApoE4 knock-in mice intraperitoneal injected with mCRP revealed that mCRP increased the expressions of phosphorylated CD31 (pCD31) and LIMA1, and facilitate the binding of pCD31 to LIMA1. mCRP combined with recombinant APOE4 protein decreased interaction of CD31 and VE-Cadherin at adherens junctions (AJs), along with altered the expression of various actin cytoskeleton proteins, causing microvasculature damage. Notably, the APOE2 protein attenuated these changes. Overall, our study demonstrates that ApoE4 responds to mCRP to disrupt the endothelial AJs which link with the actin cytoskeleton and this pathway could play a key role in the barrier dysfunction leading to AD risk.","is_dataset_classified":null,"base_score":2.302585092994046,"endowment":2.302585092994046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"39276310","pmcid":"PMC11564276","openalex_id":"https://openalex.org/W4402538358","authors":[],"funders":[{"funder_name":"National Institute on Aging","grant_id":"R01 AG054546","title":null},{"funder_name":"NIA NIH HHS","grant_id":"RF1 AG075832","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI054546","title":null},{"funder_name":"National Institutes of Health","grant_id":"1R56AG059805-01","title":"Quantitative systems pharmacology and translational study of pramlintide for Alzheimer's disease"},{"funder_name":"National Institutes of Health","grant_id":"1RF1AG075832-01A1","title":"Identification and characterization of the CD31-ApoE-mCRP pathway for Alzheimer's disease in humans."}],"total_grants":5,"fwci":2.121,"citation_percentile":0.86703215,"influential_citations":0,"citation_trend":[{"year":2024,"count":1},{"year":2025,"count":6},{"year":2026,"count":2}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"https://link.springer.com/content/pdf/10.1007/s10456-024-09946-4.pdf","host_type":"journal"},{"url":"https://link.springer.com/content/pdf/10.1007/s10456-024-09946-4.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1007/s10456-024-09946-4/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1007/s10456-024-09946-4","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/39276310","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11564276","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11564276/pdf/10456_2024_Article_9946.pdf","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC11564276","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC11564276?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.21203/rs.3.rs-3491703/v1","host_type":""},{"url":"http://dx.doi.org/10.1007/s10456-024-09946-4","host_type":""}],"fields_of_study":["Cellular Mechanics and Interactions","Blood properties and coagulation","Cell Adhesion Molecules Research","0301 basic medicine","03 medical and health sciences","Animals","Adherens Junctions","Actin Cytoskeleton","Humans","Protein Isoforms","Mice","C-Reactive Protein","Apolipoproteins E","Endothelial Cells","Alzheimer Disease","Female","Male","Brain","Apolipoprotein E4","Platelet Endothelial Cell Adhesion Molecule-1","Mice, Transgenic","Cadherins"],"mesh_terms":["Alzheimer Disease","Animals","Apolipoproteins E","Brain","C-Reactive Protein","Female","Humans","Male","Mice, Transgenic","Actin Cytoskeleton","Cadherins","Platelet Endothelial Cell Adhesion Molecule-1","Protein Isoforms","Adherens Junctions","Endothelial Cells","Mice","Apolipoprotein E4"],"keywords":["Adherens junction","Cytoskeleton","Actin cytoskeleton","Cell biology","Actin","Gene isoform","Angiogenesis","Biology","Apolipoprotein E","Medicine","Cancer research","Genetics","Cadherin","Pathology","Gene","Cell","Disease","F-actin","Endothelia","Cerebrovascular","Alzheimer’s Disease (Ad)","Apolipoprotein E (Apoe)","Monomeric C-Reactive Protein (Mcrp)","Cd31 And Pcd31","Lim Domain And Actin Binding 1 (Lima1)","Male","Original Paper","Apolipoprotein E4","Endothelial Cells","Brain","Mice, Transgenic","Adherens Junctions","Cadherins","Platelet Endothelial Cell Adhesion Molecule-1","Mice","C-Reactive Protein","Apolipoproteins E","Alzheimer Disease","Animals","Humans","Protein Isoforms","Female"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T22:06:52.443262Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}