{"doi":"10.1007/s10295-017-1997-x","title":"Discovery of a new diol-containing polyketide by heterologous expression of a silent biosynthetic gene cluster from <i>Streptomyces lavendulae</i> FRI-5","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>The genome of streptomycetes has the ability to produce many novel and potentially useful bioactive compounds, but most of which are not produced under standard laboratory cultivation conditions and are referred to as silent/cryptic secondary metabolites. Streptomyces lavendulae FRI-5 produces several types of bioactive compounds. However, this strain may also have the potential to biosynthesize more useful secondary metabolites. Here, we activated a silent biosynthetic gene cluster of an uncharacterized compound from S. lavendulae FRI-5 using heterologous expression. The engineered strain carrying the silent gene cluster produced compound 5, which was undetectable in the culture broth of S. lavendulae FRI-5. Using various spectroscopic analyses, we elucidated the chemical structure of compound 5 (named lavendiol) as a new diol-containing polyketide. The proposed assembly line of lavendiol shows a unique biosynthetic mechanism for polyketide compounds. The results of this study suggest the possibility of discovering more silent useful compounds from streptomycetes by genome mining and heterologous expression.</jats:p>","journal":"Journal of Industrial Microbiology and Biotechnology","year":2018,"id":679717,"datarank":0.5375278407684165,"base_score":3.58351893845611,"endowment":3.58351893845611,"self_citation_contribution":0.5375278407684165,"citation_network_contribution":0.0,"self_endowment_contribution":0.5375278407684165,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":35,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1573093,"name":"Shigeru Kitani","orcid":null,"position":1,"is_corresponding":false},{"id":1775963,"name":"Farah Wahidah Roslan","orcid":null,"position":2,"is_corresponding":false},{"id":1775965,"name":"Dana Ulanova","orcid":null,"position":3,"is_corresponding":false},{"id":405279,"name":"Masayoshi Arai","orcid":"0000-0003-2771-1948","position":4,"is_corresponding":false},{"id":1573106,"name":"Haruo Ikeda","orcid":null,"position":5,"is_corresponding":false},{"id":1573107,"name":"Takuya Nihira","orcid":null,"position":6,"is_corresponding":false},{"id":1775961,"name":"Ivy Grace Umadhay Pait","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Discovery of a new diol-containing polyketide by heterologous expression of a silent biosynthetic gene cluster from <i>Streptomyces lavendulae</i> FRI-5","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>The genome of streptomycetes has the ability to produce many novel and potentially useful bioactive compounds, but most of which are not produced under standard laboratory cultivation conditions and are referred to as silent/cryptic secondary metabolites. Streptomyces lavendulae FRI-5 produces several types of bioactive compounds. However, this strain may also have the potential to biosynthesize more useful secondary metabolites. Here, we activated a silent biosynthetic gene cluster of an uncharacterized compound from S. lavendulae FRI-5 using heterologous expression. The engineered strain carrying the silent gene cluster produced compound 5, which was undetectable in the culture broth of S. lavendulae FRI-5. Using various spectroscopic analyses, we elucidated the chemical structure of compound 5 (named lavendiol) as a new diol-containing polyketide. The proposed assembly line of lavendiol shows a unique biosynthetic mechanism for polyketide compounds. The results of this study suggest the possibility of discovering more silent useful compounds from streptomycetes by genome mining and heterologous expression.</jats:p>","is_dataset_classified":null,"base_score":3.58351893845611,"endowment":3.58351893845611,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29255990","pmcid":null,"openalex_id":"https://openalex.org/W2779189714","authors":[],"funders":[{"funder_name":"Japan Society for the Promotion of Science","grant_id":"JP15K07358","title":null},{"funder_name":"Japan Association for Chemical Innovation","grant_id":"the New Chemical Technology Research Encouragement Award","title":null},{"funder_name":"Ministry of Education, Culture, Sports, Science and Technology","grant_id":"scholarship","title":null}],"total_grants":3,"fwci":2.0878,"citation_percentile":0.86248357,"influential_citations":0,"citation_trend":[{"year":2018,"count":1},{"year":2019,"count":5},{"year":2020,"count":5},{"year":2021,"count":2},{"year":2022,"count":6},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":10},{"year":2026,"count":3}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/article/10.1007/s10295-017-1997-x/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s10295-017-1997-x.pdf","host_type":"publisher"},{"url":"http://academic.oup.com/jimb/article-pdf/45/2/77/36808358/jimb0077.pdf","host_type":"publisher"},{"url":"https://doi.org/10.1007/s10295-017-1997-x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29255990","host_type":"repository"}],"fields_of_study":["Microbial Natural Products and Biosynthesis","Marine Sponges and Natural Products","Synthetic Organic Chemistry Methods"],"mesh_terms":["Genes, Fungal","Streptomyces","Gene Expression","Biosynthetic Pathways","Polyketides","Secondary Metabolism"],"keywords":["Polyketide","Gene cluster","Heterologous expression","Streptomyces","Heterologous","Biology","Polyketide synthase","Gene","Genome","Strain (injury)","Biochemistry","Biosynthesis","Genetics","Bacteria","Recombinant DNA","Genome Mining","Silent Secondary Metabolites"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T13:39:29.795683Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}