{"doi":"10.1007/s101269900004","title":"Molecular Cloning of Growth Hormone Complementary DNA in Barfin Flounder (Verasper moseri)","abstract":null,"journal":"Marine Biotechnology","year":2000,"id":688070,"datarank":0.41588830833596724,"base_score":2.772588722239781,"endowment":2.772588722239781,"self_citation_contribution":0.41588830833596724,"citation_network_contribution":0.0,"self_endowment_contribution":0.41588830833596724,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":15,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1535713,"name":"Shunsuke Moriyama","orcid":null,"position":1,"is_corresponding":false},{"id":120325,"name":"Akiyoshi Takahashi","orcid":null,"position":2,"is_corresponding":false},{"id":120324,"name":"Hiroshi Kawauchi","orcid":null,"position":3,"is_corresponding":false},{"id":1797520,"name":"Punia Peyush","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Molecular Cloning of Growth Hormone Complementary DNA in Barfin Flounder (Verasper moseri)","abstract":"The olive flounder (family Paralichthidae; Paralichthys olivaceus) growth hormone (ofGH) appears to be the most derived among known growth hormones, with the deletion of 14 consecutive amino acids in the carboxy-terminal region. To ascertain if this deletion is common to all flounders, growth hormone complementary DNA of the barfin flounder (bfGH) (family Pleuronectidae; Verasper moseri) has been cloned. It was amplified by polymerase chain reaction using single-strand cDNA from the pituitary gland. Excluding the poly(A) tail, the bfGH cDNA is 919 nucleotides long and contains a 609-bp open reading frame encoding a putative signal peptide of 17 amino acids and a mature protein of 186 amino acids. Northern blot analysis detected 1.0 kb of bfGH messenger RNA in the pituitary gland, which is a reasonable value considering the poly(A) tail. The deduced amino acid sequence of bfGH has 78% identity with the sequence of ofGH. A major difference is the presence of a 14 amino acid segment (140-153) in bfGH, as in other growth hormones, suggesting that this deletion in the olive flounder occurred after the divergence of the Pleuronectoidae.","is_dataset_classified":null,"base_score":2.772588722239781,"endowment":2.772588722239781,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10804236","pmcid":null,"openalex_id":"https://openalex.org/W1596050058","authors":[],"funders":[],"total_grants":0,"fwci":1.5795,"citation_percentile":0.81971545,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2022,"count":2}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/s101269900004.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s101269900004/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1007/s101269900004","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10804236","host_type":"repository"}],"fields_of_study":["Aquaculture Nutrition and Growth","Animal Genetics and Reproduction","Reproductive biology and impacts on aquatic species"],"mesh_terms":[],"keywords":["Biology","Flounder","Cloning (programming)","Molecular cloning","Olive flounder","Zoology","Fishery","Genetics","Gene","Fish <Actinopterygii>","Complementary DNA"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-19T12:38:55.986022Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}