{"doi":"10.1007/s00792-016-0876-0","title":"Sequential formation of two branched intermediates during protein splicing of class three inteins","abstract":null,"journal":"Extremophiles","year":2017,"id":645305,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1680220,"name":"Francine Perler","orcid":null,"position":1,"is_corresponding":false},{"id":1680218,"name":"Kazuo Tori","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Sequential formation of two branched intermediates during protein splicing of class three inteins","abstract":"Inteins are the protein equivalent of introns. They are seamlessly removed during post-translational maturation of their host protein (extein). Inteins from extremophiles played a key role in understanding intein-mediated protein splicing. There are currently three classes of inteins defined by catalytic mechanism and sequence signatures. This study demonstrates splicing of three class 3 mini-inteins: Burkholderia vietnamiensis G4 Bvi IcmO intein, Mycobacterium smegmatis MC2 155 Msm DnaB-1 intein and Mycobacterium leprae strain TN Mle DnaB intein. B. vietnamiensis has a broad ecological range and remediates trichloroethene. M. smegmatis is a biofilm forming soil bacteria. Although other intein classes have only a single branched intermediate at the C-terminal splice junction, the class 3 intein reaction pathway includes two branched intermediates. The class 3 specific branched intermediate is formed by an internal cysteine, while the C-terminal branch intermediate is at a serine or threonine in all class 3 inteins except the Bvi IcmO intein, where it is a cysteine. This latter cysteine was unable to compensate for mutation of the class 3-specific internal catalytic cysteine despite the Bvi IcmO intein having an N-terminal splice junction naturally tuned for a cysteine nucleophile, demonstrating the mandatory order of branch intermediates in class 3 inteins.","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27704298","pmcid":"PMC5222942","openalex_id":"https://openalex.org/W2528848796","authors":[],"funders":[],"total_grants":0,"fwci":0.1603,"citation_percentile":0.63015425,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2020,"count":4},{"year":2022,"count":1}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://link.springer.com/content/pdf/10.1007/s00792-016-0876-0.pdf","host_type":"journal"},{"url":"https://link.springer.com/content/pdf/10.1007/s00792-016-0876-0.pdf","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s00792-016-0876-0.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s00792-016-0876-0/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00792-016-0876-0","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27704298","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5222942","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC5222942","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC5222942?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["RNA and protein synthesis mechanisms","Bacterial Genetics and Biotechnology","Monoclonal and Polyclonal Antibodies Research"],"mesh_terms":["Bacterial Proteins","Mycobacterium leprae","Protein Processing, Post-Translational","Burkholderia","Inteins"],"keywords":["Intein","Protein splicing","dnaB helicase","Biology","Genetics","RNA splicing","Biochemistry","Gene","Cysteine","Enzyme Mechanism","Branched Intermediate"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T04:37:55.512534Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}