{"doi":"10.1007/s00705-019-04283-0","title":"PB2 and hemagglutinin mutations confer a virulent phenotype on an H1N2 avian influenza virus in mice","abstract":null,"journal":"Archives of Virology","year":2019,"id":619506,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":149756,"name":"Zhiguang Ren","orcid":null,"position":1,"is_corresponding":false},{"id":149769,"name":"Yongkun Zhao","orcid":null,"position":2,"is_corresponding":false},{"id":1598730,"name":"Kaihui Cheng","orcid":null,"position":3,"is_corresponding":false},{"id":149766,"name":"Weiyang Sun","orcid":null,"position":4,"is_corresponding":false},{"id":1598731,"name":"Xinghai Zhang","orcid":null,"position":5,"is_corresponding":false},{"id":1598732,"name":"Jiaqiang Wu","orcid":null,"position":6,"is_corresponding":false},{"id":1598733,"name":"Hongbin He","orcid":null,"position":7,"is_corresponding":false},{"id":149771,"name":"Xianzhu Xia","orcid":null,"position":8,"is_corresponding":false},{"id":149768,"name":"Yuwei Gao","orcid":null,"position":9,"is_corresponding":false},{"id":1598729,"name":"Zhijun Yu","orcid":"0000-0003-4588-8800","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"PB2 and hemagglutinin mutations confer a virulent phenotype on an H1N2 avian influenza virus in mice","abstract":"We previously obtained mouse-adapted variants of H1N2 avian influenza virus that contained PB2-L134H, PB2-I647L, PB2-D701N, HA-G228S, and M1-D231N mutations. Here, we analyzed the effects of these mutations on viral pathogenicity in a mammalian model. By evaluating the virulence of mouse-adapted H1N2 variants at different generations, we found that the PB2-D701N and HA-G228S mutations both contribute to the virulence of this virus in mammals. Furthermore, we found that the PB2-D701N and HA-G228S mutations both enhance the ability of the virus to replicate in vivo and in vitro and that the PB2-D701N substitution results in an expansion of viral tissue tropism. These results suggest that the PB2-D701N mutation and the HA-G228S mutation are the major mammalian determinants of H1N2 virus. These results help us to understand more about the mechanisms by which influenza viruses adapt to mammals, and monitoring of these mutations can be used in continuous influenza surveillance to assess the pandemic potential of avian influenza virus variants.","is_dataset_classified":null,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31111259","pmcid":null,"openalex_id":"https://openalex.org/W2946588866","authors":[],"funders":[{"funder_name":"High-level Talent Projects","grant_id":"ts201511069","title":null},{"funder_name":"High-level Talent Projects","grant_id":"W03020496","title":null},{"funder_name":"Natural Science Foundation of Shandong Province","grant_id":"ZR2018QC005","title":null},{"funder_name":"the National Key Technology R&D Program","grant_id":"2013BAD12B04","title":null},{"funder_name":"National key research and development plan","grant_id":"2016YFD0500203","title":null},{"funder_name":"National key research and development plan","grant_id":"2017YFD0500100","title":null},{"funder_name":"the Construction of Subjects and Teams of Institue of Poultry Science","grant_id":"CXGC2018E11","title":null},{"funder_name":"the High-Level Talents and Innovative Team Recruitment Program of the Shandong Academy of Agricultural Sciences","grant_id":"","title":null}],"total_grants":8,"fwci":0.4401,"citation_percentile":0.63509155,"influential_citations":0,"citation_trend":[{"year":2019,"count":1},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":5},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/s00705-019-04283-0.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s00705-019-04283-0/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00705-019-04283-0","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31111259","host_type":"repository"}],"fields_of_study":["Influenza Virus Research Studies","Viral gastroenteritis research and epidemiology","Animal Disease Management and Epidemiology","Adaptation, Biological","Amino Acid Substitution","Animals","Birds","Cell Line","Dogs","Female","Hemagglutinin Glycoproteins, Influenza Virus","Influenza A Virus, H1N2 Subtype","Influenza in Birds","Madin Darby Canine Kidney Cells","Mammals","Mice","Mice, Inbred BALB C","Mutation","Orthomyxoviridae Infections","Phenotype","Viral Proteins","Virulence","Virus Replication"],"mesh_terms":["Adaptation, Biological","Animals","Birds","Cell Line","Dogs","Female","Influenza in Birds","Mammals","Mice, Inbred BALB C","Mutation","Orthomyxoviridae Infections","Phenotype","Viral Proteins","Virulence","Virus Replication","Hemagglutinin Glycoproteins, Influenza Virus","Amino Acid Substitution","Mice","Influenza A Virus, H1N2 Subtype","Madin Darby Canine Kidney Cells"],"keywords":["Biology","Virology","Virulence","Hemagglutinin (influenza)","H5N1 genetic structure","Phenotype","Medical microbiology","Virus","Influenza A virus subtype H5N1","Influenza A virus","Avian influenza virus","Gene","Genetics","Infectious disease (medical specialty)","Coronavirus disease 2019 (COVID-19)"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.26195784.v1","title":"Additional file 1 of Amino acid mutations PB1-V719M and PA-N444D combined with PB2-627K contribute to the pathogenicity of H7N9 in mice","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26195784","title":"Additional file 1 of Amino acid mutations PB1-V719M and PA-N444D combined with PB2-627K contribute to the pathogenicity of H7N9 in mice","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T07:26:39.592073Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}