{"doi":"10.1007/s00705-015-2560-6","title":"Rice grassy stunt virus nonstructural protein p5 serves as a viral suppressor of RNA silencing and interacts with nonstructural protein p3","abstract":null,"journal":"Archives of Virology","year":2015,"id":592941,"datarank":0.5195397063569125,"base_score":2.639057329615259,"endowment":2.639057329615259,"self_citation_contribution":0.3958585994422889,"citation_network_contribution":0.12368110691462363,"self_endowment_contribution":0.3958585994422889,"citer_contribution":0.12368110691462363,"corpus_percentile":null,"corpus_rank":null,"citation_count":13,"citer_count":9,"citers_with_citation_signal":6,"citers_with_endowment":6,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1517401,"name":"Xiao-juan Liu","orcid":null,"position":1,"is_corresponding":false},{"id":1517402,"name":"Kang-cheng Wu","orcid":null,"position":2,"is_corresponding":false},{"id":1517403,"name":"Lu-Ping Zheng","orcid":null,"position":3,"is_corresponding":false},{"id":1517404,"name":"Zuo-mei Ding","orcid":null,"position":4,"is_corresponding":false},{"id":108153,"name":"Fei Li","orcid":"0000-0003-0720-3129","position":5,"is_corresponding":false},{"id":540887,"name":"Peng Zou","orcid":"0000-0002-8484-5769","position":6,"is_corresponding":false},{"id":552217,"name":"Liang Yang","orcid":"0009-0007-2903-641X","position":7,"is_corresponding":false},{"id":1517405,"name":"Jian-guo Wu","orcid":null,"position":8,"is_corresponding":false},{"id":1517406,"name":"Zu-jian Wu","orcid":null,"position":9,"is_corresponding":false},{"id":1254585,"name":"Chao Zhang","orcid":"0009-0007-8631-2476","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Rice grassy stunt virus nonstructural protein p5 serves as a viral suppressor of RNA silencing and interacts with nonstructural protein p3","abstract":"Rice grassy stunt virus (RGSV), a member of the genus Tenuivirus, causes serious rice disease in Southeast Asian countries. In this study, a green fluorescent protein (GFP)-based transient expression assay was conducted to show that p5, encoded on RNA5 in the viral sense, is a viral suppressor of RNA silencing (VSR). Protein-protein interactions (PPIs) between p5 and all RGSV proteins except pC1 and pC2 were investigated using Gal4-based yeast two-hybrid (Y2H) experiments. The results demonstrated that p5 interacts with itself and with p3 encoded on RNA3 in the viral sense. p5-p5 and p5-p3 interactions were detected by bimolecular fluorescence complementation (BiFC) assay, and the p5-p3 interaction was confirmed by subcellular co-localization and co-immunoprecipitation (Co-IP) assays. Using the Y2H system, we demonstrated that the p5-p3 interaction requires both the N-terminal (amino acid residues 1 to 99) and C-terminal (amino acid residues 94 to 191) domains of p5. In addition, either p5 or p3 could enhance the pathogenicity of potato virus X (PVX) in Nicotiana benthamiana plants. A much more significant enhancement of PVX pathogenicity and accumulation was observed when p5 and p3 were expressed together. Our data also showed that RGSV p3 does not function as a VSR, and it had no effect on the VSR activity of p5 or the subcellular localization pattern of p5 in plant cells from Nicotiana benthamiana.","is_dataset_classified":null,"base_score":2.639057329615259,"endowment":2.639057329615259,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26296721","pmcid":null,"openalex_id":"https://openalex.org/W1652836276","authors":[],"funders":[{"funder_name":"National Basic Research Program 973","grant_id":"2014CB138402 and 2013CBA01403","title":null},{"funder_name":"Natural Science Foundation of China","grant_id":"31272018, 31201491 and 31171821","title":null},{"funder_name":"Natural Science Foundation of Fujian Province of China","grant_id":"2013J01089","title":null}],"total_grants":3,"fwci":1.9222,"citation_percentile":0.87144557,"influential_citations":0,"citation_trend":[{"year":2016,"count":1},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2024,"count":3},{"year":2025,"count":2},{"year":2026,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/s00705-015-2560-6.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s00705-015-2560-6/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s00705-015-2560-6","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00705-015-2560-6","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26296721","host_type":"repository"}],"fields_of_study":["Plant Virus Research Studies","Plant and Fungal Interactions Research","Plant Disease Resistance and Genetics","Plant Diseases","Protein Binding","RNA Interference","Tenuivirus","Nicotiana","Two-Hybrid System Techniques","Viral Nonstructural Proteins"],"mesh_terms":["Plant Diseases","Protein Binding","Nicotiana","Viral Nonstructural Proteins","Two-Hybrid System Techniques","Tenuivirus","RNA Interference"],"keywords":["Nicotiana benthamiana","Bimolecular fluorescence complementation","Biology","Potato virus X","Subcellular localization","Virology","Gene silencing","Immunoprecipitation","Plant virus","RNA silencing","Two-hybrid screening","Green fluorescent protein","Virus","RNA interference","RNA","Cell biology","Cytoplasm","Genetics","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-26T16:19:05.879229Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}