{"doi":"10.1007/s00428-021-03265-5","title":"Cell-of-origin classification using the Hans and Lymph2Cx algorithms in primary cutaneous large B-cell lymphomas","abstract":"Primary cutaneous diffuse large B-cell lymphoma, leg type (PCDLBCL-LT) and primary cutaneous follicle center lymphoma with a diffuse population of large cells (PCFCL-LC) are both primary cutaneous B-cell lymphomas with large-cell morphology (CLBCL) but with different clinical characteristics and behavior. In systemic diffuse large B-cell lymphoma, not otherwise specified (DLBCL-NOS), gene-expression profiling (GEP) revealed two molecular subgroups based on their cell-of-origin (COO) with prognostic significance: the germinal center B-cell-like (GCB) subtype and the activated B-cell-like (ABC) subtype. This study investigated whether COO classification is a useful tool for classification of CLBCL. For this retrospective study, 51 patients with PCDLBCL-LT and 15 patients with PCFCL-LC were analyzed for their COO according to the immunohistochemistry-based Hans algorithm and the NanoString GEP-based Lymph2Cx algorithm. In PCFCL-LC, all cases (100%) classified as GCB by both Hans and Lymph2Cx. In contrast, COO classification in PCDLBCL-LT was heterogeneous. Using Hans, 75% of the PCDLBCL-LT patients classified as non-GCB and 25% as GCB, while Lymph2Cx classified only 18% as ABC, 43% as unclassified/intermediate, and 39% as GCB. These COO subgroups did not differ in the expression of BCL2 and IgM, mutations in MYD88 and/or CD79B, loss of CDKN2A, or survival. In conclusion, PCFCL-LC uniformly classified as GCB, while PCDLBCL-LT classified along the COO spectrum of DLBCL-NOS using the Hans and Lymph2Cx algorithms. In contrast to DLBCL-NOS, the clinical relevance of COO classification in CLBCL using these algorithms has limitations and cannot be used as an alternative for the current multiparameter approach in differentiation of PCDLBCL-LT and PCFCL-LC.","journal":"Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin","year":2022,"id":256066,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9606,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":729870,"name":"Ruben A. L. de Groen","orcid":"0000-0002-2998-2066","position":1,"is_corresponding":false},{"id":291904,"name":"Rein Willemze","orcid":"0000-0002-4561-7616","position":2,"is_corresponding":false},{"id":729874,"name":"Patty M. Jansen","orcid":"0000-0002-2361-9303","position":3,"is_corresponding":false},{"id":291896,"name":"Koen D. Quint","orcid":"0000-0002-3267-5630","position":4,"is_corresponding":false},{"id":729872,"name":"Tom van Wezel","orcid":"0000-0001-5773-7730","position":5,"is_corresponding":false},{"id":729871,"name":"Ronald van Eijk","orcid":"0000-0001-7955-6295","position":6,"is_corresponding":false},{"id":729873,"name":"Dina Ruano","orcid":"0000-0002-8231-3398","position":7,"is_corresponding":false},{"id":904926,"name":"Cornelis P. Tensen","orcid":"0000-0002-5325-1888","position":8,"is_corresponding":false},{"id":291890,"name":"Esther Hauben","orcid":"0000-0001-8688-9231","position":9,"is_corresponding":false},{"id":627779,"name":"Feng Jung Sherida H. Woei-A-Jin","orcid":"0000-0001-7840-6156","position":10,"is_corresponding":false},{"id":905402,"name":"Anne Marie Busschots","orcid":null,"position":11,"is_corresponding":false},{"id":98655,"name":"Anke van den Berg","orcid":"0000-0002-8894-2638","position":12,"is_corresponding":false},{"id":729878,"name":"Arjan Diepstra","orcid":"0000-0001-9239-1050","position":13,"is_corresponding":false},{"id":291858,"name":"Maarten H. Vermeer","orcid":"0000-0002-5872-4613","position":14,"is_corresponding":false},{"id":729885,"name":"Joost S.P. Vermaat","orcid":"0000-0002-1628-6256","position":15,"is_corresponding":false},{"id":904925,"name":"Anne M.R. Schrader","orcid":"0000-0003-3483-0558","position":0,"is_corresponding":true}],"reference_count":39,"raw_metadata":null,"created_at":"2026-07-19T00:25:21.336200Z","pmid":"35028710","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}