{"doi":"10.1007/s004240100587","title":"Hypoxia/fatigue-induced degradation of troponin I and troponin C: new insights into physiologic muscle fatigue","abstract":null,"journal":"Pfl�gers Archiv European Journal of Physiology","year":2001,"id":690088,"datarank":0.6010999777848708,"base_score":4.007333185232471,"endowment":4.007333185232471,"self_citation_contribution":0.6010999777848708,"citation_network_contribution":0.0,"self_endowment_contribution":0.6010999777848708,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":54,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1802857,"name":"Sheila Van Leyen","orcid":null,"position":1,"is_corresponding":false},{"id":787277,"name":"Leticia Brotto","orcid":null,"position":2,"is_corresponding":false},{"id":171127,"name":"Jian-Ping Jin","orcid":null,"position":3,"is_corresponding":false},{"id":1802859,"name":"Christopher Nosek","orcid":null,"position":4,"is_corresponding":false},{"id":1802860,"name":"Thomas Nosek","orcid":null,"position":5,"is_corresponding":false},{"id":1802856,"name":"Marco de Paula Brotto","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Hypoxia/fatigue-induced degradation of troponin I and troponin C: new insights into physiologic muscle fatigue","abstract":"Conditions such as respiratory failure and cardiopulmonary arrest can expose the diaphragm to hypoxemia. In skeletal muscles, fatiguing stimulation renders muscles hypoxic, which has long been known to dramatically reduce muscle function. We have previously demonstrated that fatiguing stimulation under hypoxic conditions disrupts both the excitation-contraction coupling (ECC) process and the isometric contractile properties (ICP) in intact diaphragm muscle strips and the contractile properties of skinned fibers isolated from these muscles. Here we have analyzed the effects of intermittent fatiguing stimulation on specific muscle proteins in muscle strips from mouse diaphragms that have been exposed to hypoxia. We report for the first time that the effects of hypoxia-fatigue, namely to decrease maximal tetanic force, maximal calcium-activated force and calcium sensitivity of the mouse diaphragm muscle, are associated with the degradation of troponins TnI and TnC (Western blot analysis). The concentrations of TnT and actin did not change under these same conditions. Because troponins are integrally involved in regulating the interaction between actin and myosin during the cross-bridge cycle, the degradation of TnI and TnC may explain the effects of hypoxia-fatigue on the ICP. This interpretation is supported by the observations that extraction of troponins from control skinned fibers mimics the effects of hypoxia-fatigue on contractile function and that incorporation of native troponins into fibers isolated from hypoxic-fatigued muscles partially restores function.","is_dataset_classified":null,"base_score":4.007333185232471,"endowment":4.007333185232471,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"11512030","pmcid":null,"openalex_id":"https://openalex.org/W2068677425","authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"HL 60304","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"T32 HL 07653","title":null},{"funder_name":"National Institutes of Health","grant_id":"1R01HL060304-01","title":"DIAPHRAGMATIC FATIGUE, AGING AND REACTIVE OXYGEN SPECIES"}],"total_grants":3,"fwci":0.8899,"citation_percentile":0.72241611,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":1},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":4},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2021,"count":3},{"year":2022,"count":1},{"year":2023,"count":8}],"oa_status":"closed","license":"Springer TDM","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/s004240100587.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s004240100587/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s004240100587","host_type":"publisher"},{"url":"https://doi.org/10.1007/s004240100587","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/11512030","host_type":"repository"},{"url":"https://dx.doi.org/10.1007/s004240100587","host_type":""}],"fields_of_study":["Muscle Physiology and Disorders","Cardiovascular Effects of Exercise","Genetic Neurodegenerative Diseases","0301 basic medicine","0303 health sciences","03 medical and health sciences","Animals","Cell Hypoxia","Electric Stimulation","Immunoblotting","In Vitro Techniques","Mice","Muscle Contraction","Muscle Fatigue","Muscle Fibers, Skeletal","Muscle, Skeletal","Recombinant Proteins","Troponin C","Troponin I"],"mesh_terms":["Animals","Electric Stimulation","Muscle Contraction","Recombinant Proteins","Immunoblotting","Cell Hypoxia","Muscle, Skeletal","Muscle Fibers, Skeletal","Muscle Fatigue","Troponin C","Troponin I","Mice","In Vitro Techniques"],"keywords":["Troponin","Isometric exercise","Muscle fatigue","Troponin C","Myosin","Internal medicine","Troponin I","Stimulation","Muscle contraction","Hypoxia (environmental)","Actin","Contraction (grammar)","Cardiology","Skeletal muscle","Chemistry","Anatomy","Biology","Medicine","Biochemistry","Electromyography","Neuroscience","Oxygen","Immunoblotting","Muscle Fibers, Skeletal","In Vitro Techniques","Cell Hypoxia","Electric Stimulation","Recombinant Proteins","Mice","Animals","Muscle, Skeletal"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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