{"doi":"10.1007/s00418-014-1249-3","title":"Correlative video-light–electron microscopy: development, impact and perspectives","abstract":null,"journal":"Histochemistry and Cell Biology","year":2014,"id":682673,"datarank":0.3596842909197557,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.0,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1783438,"name":"Seetharaman Parashuraman","orcid":null,"position":1,"is_corresponding":false},{"id":168532,"name":"Alberto Luini","orcid":null,"position":2,"is_corresponding":false},{"id":1016657,"name":"Riccardo Rizzo","orcid":"0000-0001-8297-6776","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Correlative video-light–electron microscopy: development, impact and perspectives","abstract":"Green fluorescent protein (GFP)-based video microscopy can provide profound insight into biological processes by generating information on the 'history,' or dynamics, of the cellular structures involved in such processes in live cells. A crucial limitation of this approach, however, is that many such structures may not be resolved by light microscopy. Like more recent super-resolution techniques, correlative video-light-electron microscopy (CLEM) was developed to overcome this limitation. CLEM integrates GFP-based video microscopy and electron microscopy through a series of ancillary techniques, such as proper fixation, hybrid labeling and retracing, and so provides sufficient resolution as well as, crucially, cellular 'context' to the fluorescent dynamic structures of interest. CLEM 'multiplies' the power of video microscopy and is having an important impact in several areas cell and developmental biology. Here, we discuss potential, limitations and perspectives of correlative approaches aimed at integrating the unique insight generated by video microscopy with information from other forms of imaging.","is_dataset_classified":null,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25030356","pmcid":null,"openalex_id":"https://openalex.org/W2136557934","authors":[],"funders":[{"funder_name":"Telethon","grant_id":"TGM11S01","title":null},{"funder_name":"Telethon","grant_id":"TGM16S01","title":null}],"total_grants":2,"fwci":1.7907,"citation_percentile":0.87362594,"influential_citations":0,"citation_trend":[{"year":2015,"count":2},{"year":2016,"count":2},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":3},{"year":2022,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/s00418-014-1249-3.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s00418-014-1249-3/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s00418-014-1249-3","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00418-014-1249-3","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25030356","host_type":"repository"}],"fields_of_study":["Advanced Fluorescence Microscopy Techniques","Advanced Electron Microscopy Techniques and Applications","Near-Field Optical Microscopy","Engineering","Biology","Materials Science"],"mesh_terms":["Microscopy, Electron","Microscopy, Fluorescence","Staining and Labeling","Tissue Fixation","Microscopy, Video","Green Fluorescent Proteins"],"keywords":["Microscopy","Correlative","Live cell imaging","Video microscopy","Context (archaeology)","Electron microscope","Fluorescence microscope","Green fluorescent protein","Super-resolution microscopy","Biology","Resolution (logic)","Nanotechnology","Cell biology","Computer science","Fluorescence","Artificial intelligence","Optics","Scanning confocal electron microscopy","Physics","Materials science","Cell","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T21:04:39.714599Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}