{"doi":"10.1007/s00418-005-0088-7","title":"Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting","abstract":null,"journal":"Histochemistry and Cell Biology","year":2006,"id":613678,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1581079,"name":"Yme Velden","orcid":null,"position":1,"is_corresponding":false},{"id":1581081,"name":"Wim Leeuw","orcid":null,"position":2,"is_corresponding":false},{"id":1581082,"name":"Julio Mateos-Langerak","orcid":null,"position":3,"is_corresponding":false},{"id":289200,"name":"Andrew S. Belmont","orcid":"0000-0002-6540-0801","position":4,"is_corresponding":false},{"id":1581083,"name":"Roel Driel","orcid":null,"position":5,"is_corresponding":false},{"id":1581085,"name":"Pernette J. Verschure","orcid":null,"position":6,"is_corresponding":false},{"id":1581077,"name":"Maartje C. Brink","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting","abstract":"Packaging of the eukaryotic genome into higher order chromatin structures is tightly related to gene expression. Pericentromeric heterochromatin is typified by accumulations of heterochromatin protein 1 (HP1), methylation of histone H3 at lysine 9 (MeH3K9) and global histone deacetylation. HP1 interacts with chromatin by binding to MeH3K9 through the chromodomain (CD). HP1 dimerizes with itself and binds a variety of proteins through its chromoshadow domain. We have analyzed at the single cell level whether HP1 lacking its functional CD is able to induce heterochromatinization in vivo. We used a lac-operator array-based system in mammalian cells to target EGFP-lac repressor tagged truncated HP1alpha and HP1beta to a lac operator containing gene-amplified chromosome region in living cells. After targeting truncated HP1alpha or HP1beta we observe enhanced tri-MeH3K9 and recruitment of endogenous HP1alpha and HP1beta to the chromosome region. We show that CD-less HP1alpha can induce chromatin condensation, whereas the effect of truncated HP1beta is less pronounced. Our results demonstrate that after lac repressor-mediated targeting, HP1alpha and HP1beta without a functional CD are able to induce heterochromatinization.","is_dataset_classified":null,"base_score":2.70805020110221,"endowment":2.70805020110221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16283356","pmcid":null,"openalex_id":"https://openalex.org/W2132598114","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM042516","title":null}],"total_grants":1,"fwci":0.8311,"citation_percentile":0.70618287,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2016,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/s00418-005-0088-7.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s00418-005-0088-7/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s00418-005-0088-7","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00418-005-0088-7","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16283356","host_type":"repository"},{"url":"https://handle.uba.uva.nl/personal/pure/en/publications/truncated-hp1-lacking-a-functional-chromodomain-induces-heterochromatinization-upon-in-vivo-targeting(447a78cd-47f3-4b3f-9e6e-8c08fac01279).html","host_type":"repository"},{"url":"https://ir.cwi.nl/pub/15601","host_type":"repository"},{"url":"https://dare.uva.nl/personal/pure/en/publications/truncated-hp1-lacking-a-functional-chromodomain-induces-heterochromatinization-upon-in-vivo-targeting(447a78cd-47f3-4b3f-9e6e-8c08fac01279).html","host_type":"repository"}],"fields_of_study":["Genomics and Chromatin Dynamics","Epigenetics and DNA Methylation","Protein Degradation and Inhibitors"],"mesh_terms":["Chromobox Protein Homolog 5","Animals","Chromatin","Chromosomal Proteins, Non-Histone","Fluorescent Antibody Technique","Cricetinae","Heterochromatin","Histones","Humans","Image Interpretation, Computer-Assisted","Lysine","Methylation","Plasmids","CHO Cells","Microscopy, Confocal"],"keywords":["Chromodomain","Heterochromatin protein 1","Heterochromatin","Chromatin","Histone H3","Biology","Histone","Cell biology","Molecular biology","Genetics","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T08:55:26.900556Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}