{"doi":"10.1007/s00294-004-0546-0","title":"Use of bimolecular fluorescence complementation to demonstrate transcription factor interaction in nuclei of living cells from the filamentous fungus Acremonium chrysogenum","abstract":null,"journal":"Current Genetics","year":2005,"id":590755,"datarank":3.98914697829455,"base_score":4.248495242049359,"endowment":4.248495242049359,"self_citation_contribution":0.637274286307404,"citation_network_contribution":3.351872691987146,"self_endowment_contribution":0.637274286307404,"citer_contribution":3.351872691987146,"corpus_percentile":null,"corpus_rank":null,"citation_count":69,"citer_count":63,"citers_with_citation_signal":59,"citers_with_endowment":59,"datacite_reuse_total":2,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":819170,"name":"Ulrich Kück","orcid":"0000-0002-2998-8230","position":1,"is_corresponding":false},{"id":1511395,"name":"Birgit Hoff","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Use of bimolecular fluorescence complementation to demonstrate transcription factor interaction in nuclei of living cells from the filamentous fungus Acremonium chrysogenum","abstract":"Using bimolecular fluorescence complementation assays, we were able to demonstrate protein-protein interaction of the transcription factors AcFKH1 and CPCR1 in living cells from the filamentous fungus Acremonium chrysogenum. This was accomplished by splitting the gene for the enhanced yellow fluorescent protein (EYFP) into two parts encoding the N- and C-terminus. Both fragments were fused to different gene derivatives of the fungal transcription factors. The recombinant plasmids were used to generate transgenic fungal strains for subsequent confocal laser microscopy. Only when the full-length transcription factors were fused to EYFP fragments yellow fluorescence was observed due to the bimolecular complementation of both chimeric proteins. The nuclear localization of the protein-protein interaction was verified by staining fungal cells with the nucleic acid dye TOTO-3.","is_dataset_classified":null,"base_score":4.248495242049359,"endowment":4.248495242049359,"datacite_reuse_total":2,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15688253","pmcid":null,"openalex_id":"https://openalex.org/W1981891811","authors":[],"funders":[],"total_grants":0,"fwci":5.3206,"citation_percentile":0.94394904,"influential_citations":0,"citation_trend":[{"year":2012,"count":8},{"year":2013,"count":4},{"year":2014,"count":4},{"year":2015,"count":3},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2018,"count":4},{"year":2020,"count":3},{"year":2021,"count":1},{"year":2023,"count":2}],"oa_status":"closed","license":"https://www.springernature.com/gp/researchers/text-and-data-mining","oa_locations":[{"url":"https://link.springer.com/content/pdf/10.1007/s00294-004-0546-0.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1007/s00294-004-0546-0","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s00294-004-0546-0","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00294-004-0546-0","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15688253","host_type":"repository"}],"fields_of_study":["Advanced Fluorescence Microscopy Techniques","Photosynthetic Processes and Mechanisms","14-3-3 protein interactions","Acremonium","Base Sequence","Cell Nucleus","DNA Primers","Microscopy, Fluorescence","Plasmids","Protein Binding","Subcellular Fractions","Transcription Factors"],"mesh_terms":["Acremonium","Base Sequence","Cell Nucleus","Microscopy, Fluorescence","Plasmids","Protein Binding","Subcellular Fractions","Transcription Factors","DNA Primers"],"keywords":["Bimolecular fluorescence complementation","Biology","Complementation","Protein-fragment complementation assay","Fusion protein","Yellow fluorescent protein","Transcription (linguistics)","Transcription factor","Green fluorescent protein","Fluorescence","Recombinant DNA","Molecular biology","Gene","Förster resonance energy transfer","Nucleic acid","Fluorescence microscope","Biochemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[{"doi":"10.6084/m9.figshare.13474189.v1","title":"Additional file 1 of Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.13474189","title":"Additional file 1 of Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-25T11:58:52.604622Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}