{"doi":"10.1007/s00284-009-9461-4","title":"Transcriptional Analysis of the Lysine-Responsive and Riboswitch-Regulated lysC Gene of Bacillus subtilis","abstract":null,"journal":"Current Microbiology","year":2009,"id":621522,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1605070,"name":"Wolfgang Schumann","orcid":null,"position":1,"is_corresponding":false},{"id":1605069,"name":"Trang Thi Phuong Phan","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Transcriptional Analysis of the Lysine-Responsive and Riboswitch-Regulated lysC Gene of Bacillus subtilis","abstract":"About 2% of the Bacillus subtilis genes are subject to regulation by riboswitch-controlled mechanisms. One of them is the L-lysine-dependent lysC gene which is turned on when the L-lysine concentration within the cytoplasm is low. In the presence of a high L-lysine concentration, only a 0.27-kb transcript is synthesized representing the riboswitch due to transcription termination. When the L-lysine concentration is low, the full-length 1.6-kb transcript is produced due to transcription anti-termination. Here, we show for the first time that even under conditions of transcription anti-termination the truncated form of the RNA is still predominant. This 0.27-kb transcript is neither the result of enhanced stability nor does it result from processing of the full-length transcript. When the region coding for the transcription terminator was removed, the riboswitch RNA failed to be produced. These data were confirmed by analysis of a transcriptional fusion between the promoter-riboswitch region of lysC with and without a functional transcriptional terminator and the lacZ reporter gene. The putative function(s) of the riboswitch under conditions of low L-lysine concentration is discussed.","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19636616","pmcid":null,"openalex_id":"https://openalex.org/W2151429804","authors":[],"funders":[],"total_grants":0,"fwci":0.5039,"citation_percentile":0.65148119,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":1},{"year":2017,"count":1},{"year":2018,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/s00284-009-9461-4.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s00284-009-9461-4/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s00284-009-9461-4","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00284-009-9461-4","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/19636616","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","Bacterial Genetics and Biotechnology","Bacteriophages and microbial interactions"],"mesh_terms":["Bacillus subtilis","beta-Galactosidase","Lysine","RNA, Bacterial","RNA, Messenger","Gene Expression Regulation, Bacterial","Genes, Reporter","Artificial Gene Fusion","Gene Expression Profiling"],"keywords":["Riboswitch","Terminator (solar)","Bacillus subtilis","Transcription (linguistics)","Biology","RNA","Lysine","Gene","Reporter gene","Molecular biology","Transcriptional regulation","Gene expression","Genetics","Non-coding RNA","Amino acid"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T14:54:15.433188Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}