{"doi":"10.1007/s00284-005-4543-4","title":"Construction, Expression, and Characterization of a Thermostable Xylanase","abstract":null,"journal":"Current Microbiology","year":2005,"id":666196,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1739735,"name":"Jian-Yi Sun","orcid":null,"position":1,"is_corresponding":false},{"id":1739734,"name":"Xiao-Yan Weng","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Construction, Expression, and Characterization of a Thermostable Xylanase","abstract":"A hybrid gene, btx, encoding a thermostable xylanase, Btx, was constructed by substituting the 31 N-terminal amino acid residues of the Thermomonospora fusca xylanase A (TfxA) for the corresponding region of 22 amino acid residues of the Bacillus subtilis xylanase A (BsxA). The btx gene was expressed in Escherichia coli BL21. The halo size produced by xylanase Btx on a Remanzol brilliant blue R (RBB) xylan plate at 60 degrees C and pH 6.0 was larger than those of BsxA and TfxA. The molecular weight of Btx was 22 kDa. Temperature and pH optima for Btx were at 50-60 degrees C and 6.0, respectively. Btx showed activity over 80% over a pH range of 5.0-9.0, which was wider than that of BsxA, and was also more acid-resistant than TfxA. Btx exhibited significant thermostability compared with BsxA. The results show the importance of the N-terminal sequence of TfxA in thermostability.","is_dataset_classified":null,"base_score":2.70805020110221,"endowment":2.70805020110221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"16086105","pmcid":null,"openalex_id":"https://openalex.org/W2074987689","authors":[],"funders":[],"total_grants":0,"fwci":1.0097,"citation_percentile":0.75744983,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":1},{"year":2014,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/s00284-005-4543-4.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s00284-005-4543-4/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s00284-005-4543-4","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00284-005-4543-4","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/16086105","host_type":"repository"}],"fields_of_study":["Biofuel production and bioconversion","Enzyme Catalysis and Immobilization","Studies on Chitinases and Chitosanases","Actinomycetales","Amino Acid Sequence","Bacillus subtilis","Endo-1,4-beta Xylanases","Escherichia coli","Gene Expression","Genes, Bacterial","Hybridization, Genetic","Hydrogen-Ion Concentration","Molecular Sequence Data","Molecular Weight","Recombinant Proteins","Sequence Alignment","Temperature","Xylans"],"mesh_terms":["Actinomycetales","Amino Acid Sequence","Bacillus subtilis","Escherichia coli","Genes, Bacterial","Hybridization, Genetic","Hydrogen-Ion Concentration","Molecular Sequence Data","Molecular Weight","Recombinant Proteins","Temperature","Xylans","Gene Expression","Sequence Alignment","Endo-1,4-beta Xylanases"],"keywords":["Thermostability","Xylanase","Escherichia coli","Biochemistry","Bacillus subtilis","Amino acid","Biology","Xylan","Gene","Chemistry","Enzyme","Bacteria","Genetics"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T13:38:50.984546Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}