{"doi":"10.1007/s00280-010-1302-4","title":"The histone deacetylase inhibitor vorinostat induces calreticulin exposure in childhood brain tumour cells in vitro","abstract":null,"journal":"Cancer Chemotherapy and Pharmacology","year":2010,"id":633502,"datarank":0.5050943744979712,"base_score":3.367295829986474,"endowment":3.367295829986474,"self_citation_contribution":0.5050943744979712,"citation_network_contribution":0.0,"self_endowment_contribution":0.5050943744979712,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":28,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1642577,"name":"Stephanie Greßmann","orcid":null,"position":1,"is_corresponding":false},{"id":1642578,"name":"Sabine Becker","orcid":null,"position":2,"is_corresponding":false},{"id":1642579,"name":"Susan Wittig","orcid":null,"position":3,"is_corresponding":false},{"id":1642580,"name":"Mareike Schmudde","orcid":null,"position":4,"is_corresponding":false},{"id":1642581,"name":"James F. Beck","orcid":null,"position":5,"is_corresponding":false},{"id":1642576,"name":"Jürgen Sonnemann","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The histone deacetylase inhibitor vorinostat induces calreticulin exposure in childhood brain tumour cells in vitro","abstract":"<h4>Purpose</h4>It has recently been recognised that anticancer chemotherapy can elicit an immunogenic form of apoptosis characterised by the exposure of calreticulin (CRT) on the surface of dying tumour cells, entailing an immune response that contributes to the therapeutic outcome. CRT exposure has been found to be induced by anthracyclins and oxaliplatin, but not by other proapoptotic antineoplastic agents including etoposide, camptothecin and cisplatin. In this study, we examined the histone deacetylase inhibitor vorinostat for its capability to stimulate CRT exposure in tumour cells.<h4>Methods</h4>Childhood tumour cells, i.e. the brain tumour cell lines PFSK and DAOY and the Ewing's sarcoma cell line CADO-ES-1, were treated with vorinostat, and CRT exposure was determined by flow cytometric analysis of CRT immunofluorescence. Combination effects of vorinostat/TRAIL and vorinostat/bortezomib were also assessed.<h4>Results</h4>Vorinostat treatment induced CRT exposure in PFSK and DAOY cells, but not in caspase-8-deficient CADO-ES-1 cells. CRT exposure could be prevented by the pan-caspase inhibitor z-VAD-fmk and by brefeldin A, an inhibitor of Golgi-mediated transport.<h4>Conclusion</h4>Vorinostat has the capacity to elicit CRT exposure, suggesting its usefulness as immunogenic antitumour agent.","is_dataset_classified":null,"base_score":3.367295829986474,"endowment":3.367295829986474,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20221600","pmcid":null,"openalex_id":"https://openalex.org/W2043322963","authors":[],"funders":[],"total_grants":0,"fwci":0.7471,"citation_percentile":0.68969546,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2013,"count":4},{"year":2014,"count":2},{"year":2015,"count":1},{"year":2016,"count":6},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2020,"count":3},{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":1}],"oa_status":"green","license":"other-oa","oa_locations":[{"url":"https://hal.science/hal-00575248","host_type":"repository"},{"url":"https://hal.science/hal-00575248","host_type":"repository"},{"url":"http://link.springer.com/content/pdf/10.1007/s00280-010-1302-4.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s00280-010-1302-4/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s00280-010-1302-4","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00280-010-1302-4","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20221600","host_type":"repository"},{"url":"http://hdl.handle.net/2262/53195","host_type":"repository"}],"fields_of_study":["Histone Deacetylase Inhibitors Research","Endoplasmic Reticulum Stress and Disease","Autophagy in Disease and Therapy"],"mesh_terms":["Bortezomib","Vorinostat","Antineoplastic Agents","Boronic Acids","Brain Neoplasms","Child","Flow Cytometry","Fluorescent Antibody Technique","Humans","Hydroxamic Acids","Pyrazines","Calreticulin","Cell Line, Tumor","Caspase 8","Histone Deacetylase Inhibitors"],"keywords":["Vorinostat","Histone deacetylase inhibitor","Cancer research","Calreticulin","Histone deacetylase","Immunogenic cell death","Biology","Camptothecin","Apoptosis","Pharmacology","Programmed cell death","Cell biology","Histone","Endoplasmic reticulum"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-06T12:11:03.368183Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}