{"doi":"10.1007/s00280-008-0686-x","title":"BRCA1 transcriptional activity is enhanced by interactions between its AD1 domain and AhR","abstract":null,"journal":"Cancer Chemotherapy and Pharmacology","year":2008,"id":603502,"datarank":0.4335557636844247,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"self_citation_contribution":0.4335557636844247,"citation_network_contribution":0.0,"self_endowment_contribution":0.4335557636844247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":17,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1233168,"name":"Hee Jeong Kim","orcid":"0000-0002-4509-953X","position":1,"is_corresponding":false},{"id":1548127,"name":"Chi-Heum Cho","orcid":null,"position":2,"is_corresponding":false},{"id":341854,"name":"Yanfen Hu","orcid":"0000-0001-9595-1631","position":3,"is_corresponding":false},{"id":503630,"name":"Rong Li","orcid":"0000-0002-7910-6494","position":4,"is_corresponding":false},{"id":1548130,"name":"Insoo Bae","orcid":null,"position":5,"is_corresponding":false},{"id":1548124,"name":"Hyo Jin Kang","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"BRCA1 transcriptional activity is enhanced by interactions between its AD1 domain and AhR","abstract":"<h4>Purpose</h4>We previously reported that BRCA1 interacts with aryl hydrocarbon receptor nuclear translocator (ARNT) and that this interaction affects TCDD-induced CYP1A1 gene expression (Kang et al., J Biol Chem 281:14654-14662, 2006). In this study we continue this investigation and begin to define the significance of this interaction for the regulation of stress-induced transcription.<h4>Methods</h4>Immunoprecipitations (IPs), western blot (WB) analysis, GST pull-down assays and promoter reporter assays were used to investigate whether the aryl hydrocarbon receptor (AhR) can regulate transcription that is dependent on the activation domain 1 (AD1) domain of BRCA1.<h4>Results</h4>We show that AhR, a transcription factor, can bind specifically to AD1 in the C-terminal region of BRCA1 and affect BRCA1's ability to regulate transcription activity. We found that xenobiotics that positively and negatively affect AhR's activity as a transcription factor (e.g., dioxin and alpha-naphthoflavone, respectively), have similar effects on AhR's ability to affect AD1-domain-dependent transcription. These physical and functional AhR-AD1 interactions may require the coiled-coil motif in AD1 because point-mutations in this motif reduce these interactions.<h4>Conclusion</h4>Xenobiotic-activated AhR can function in two ways, as a component of the AhR/ARNT transcription factor and a regulator of AD1-dependent transcription. Consequently, BRCA1 has two distinct mechanisms for sensing xenobiotics and regulating AhR-dependent stress responses to these xenobiotics. We speculate that the normal functioning of this interaction could play a role in BRCA1's tumor suppressing ability.","is_dataset_classified":null,"base_score":2.8903717578961645,"endowment":2.8903717578961645,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"18259752","pmcid":"PMC2702208","openalex_id":"https://openalex.org/W2137735293","authors":[],"funders":[{"funder_name":"NIEHS NIH HHS","grant_id":"ES01440-01","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA118578","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA118578","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA093506","title":null},{"funder_name":"NCI NIH HHS","grant_id":"CA93506","title":null}],"total_grants":5,"fwci":1.7599,"citation_percentile":0.82398825,"influential_citations":0,"citation_trend":[{"year":2013,"count":2},{"year":2014,"count":1},{"year":2016,"count":1},{"year":2017,"count":1},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2023,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":"cc-by-nc-nd","oa_locations":[{"url":"http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35295","host_type":"repository"},{"url":"http://kumel.medlib.dsmc.or.kr/handle/2015.oak/35295","host_type":"repository"},{"url":"http://link.springer.com/content/pdf/10.1007/s00280-008-0686-x.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s00280-008-0686-x/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s00280-008-0686-x","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00280-008-0686-x","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/18259752","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2702208","host_type":"repository"}],"fields_of_study":["Toxic Organic Pollutants Impact","Carcinogens and Genotoxicity Assessment","Effects and risks of endocrine disrupting chemicals"],"mesh_terms":["Polychlorinated Dibenzodioxins","Benzoflavones","Binding, Competitive","Humans","Neoplasm Proteins","Protein Binding","Transcription, Genetic","Gene Expression Regulation, Neoplastic","Proto-Oncogene Proteins c-jun","Protein Structure, Tertiary","Genes, Reporter","Receptors, Aryl Hydrocarbon","BRCA1 Protein","Protein Interaction Mapping","Cell Line, Tumor"],"keywords":["Aryl hydrocarbon receptor","Aryl hydrocarbon receptor nuclear translocator","Transcription factor","Transcription (linguistics)","Cell biology","Biology","Xenobiotic","Transcriptional regulation","Chemistry","Gene","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T22:06:45.690993Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}