{"doi":"10.1007/s00262-021-03101-4","title":"Efficient adoptive transfer of autologous modified B cells: a new humanized platform mouse model for testing B cells reprogramming therapies","abstract":"<jats:title>Abstract</jats:title><jats:p>Here, we report a novel experimental setup to perform adoptive transfer of gene-edited B cells using humanized immune system mice by infusing autologous HIS mouse-derived human B cells “educated” in a murine context and thus rendered tolerant to the host. The present approach presents two advantages over the conventional humanized PBMC mouse models: (i) it circumvents the risk of xenogeneic graft-versus-host reaction and (ii) it mimics more closely human immune responses, thus favoring clinical translation. We show that the frequencies and numbers of transduced B cells in recipient’s spleens one week post-transfer are within the range of the size of the pre-immune B cell population specific for a given protein antigen in the mouse. They are also compatible with the B cell numbers required to elicit a sizeable immune response upon immunization. Altogether, our findings pave the way for future studies aiming at assessing therapeutic interventions involving B cell reprogramming for instance by an antibody transgene in a “humanized” hematopoietic setting.</jats:p>","journal":"Cancer Immunology, Immunotherapy","year":2022,"id":652890,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":846452,"name":"Emilie Laurent","orcid":"0000-0002-8993-8905","position":1,"is_corresponding":false},{"id":1703372,"name":"Didier Nègre","orcid":null,"position":2,"is_corresponding":false},{"id":681091,"name":"Caroline Costa","orcid":null,"position":3,"is_corresponding":false},{"id":1703373,"name":"Véronique Pierre","orcid":null,"position":4,"is_corresponding":false},{"id":459111,"name":"Thierry Defrance","orcid":"0000-0002-6943-358X","position":5,"is_corresponding":false},{"id":605883,"name":"François‐Loïc Cosset","orcid":"0000-0001-8842-3726","position":6,"is_corresponding":false},{"id":578481,"name":"Floriane Fusil","orcid":"0000-0002-1619-7333","position":7,"is_corresponding":false},{"id":1702990,"name":"Audrey Page","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Efficient adoptive transfer of autologous modified B cells: a new humanized platform mouse model for testing B cells reprogramming therapies","abstract":"<jats:title>Abstract</jats:title><jats:p>Here, we report a novel experimental setup to perform adoptive transfer of gene-edited B cells using humanized immune system mice by infusing autologous HIS mouse-derived human B cells “educated” in a murine context and thus rendered tolerant to the host. The present approach presents two advantages over the conventional humanized PBMC mouse models: (i) it circumvents the risk of xenogeneic graft-versus-host reaction and (ii) it mimics more closely human immune responses, thus favoring clinical translation. We show that the frequencies and numbers of transduced B cells in recipient’s spleens one week post-transfer are within the range of the size of the pre-immune B cell population specific for a given protein antigen in the mouse. They are also compatible with the B cell numbers required to elicit a sizeable immune response upon immunization. Altogether, our findings pave the way for future studies aiming at assessing therapeutic interventions involving B cell reprogramming for instance by an antibody transgene in a “humanized” hematopoietic setting.</jats:p>","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"34748076","pmcid":"PMC9188505","openalex_id":"https://openalex.org/W3212763058","authors":[],"funders":[{"funder_name":"French National Research Agency (ANR)","grant_id":"ANR-11-LABX-0048","title":null},{"funder_name":"French National Research Agency (ANR)","grant_id":"ANR-11-IDEX-0007","title":null},{"funder_name":"ANRS","grant_id":"","title":null},{"funder_name":"Janssen Horizon","grant_id":"","title":null},{"funder_name":"LabEx Ecofect","grant_id":"","title":null}],"total_grants":5,"fwci":0.9086,"citation_percentile":0.77184918,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2023,"count":3},{"year":2024,"count":2},{"year":2025,"count":2}],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://link.springer.com/content/pdf/10.1007/s00262-021-03101-4.pdf","host_type":"journal"},{"url":"https://link.springer.com/content/pdf/10.1007/s00262-021-03101-4.pdf","host_type":"publisher"},{"url":"https://link.springer.com/article/10.1007/s00262-021-03101-4/fulltext.html","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00262-021-03101-4","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/34748076","host_type":"repository"},{"url":"https://hal.science/hal-03428459","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9188505","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9188505","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9188505?pdf=render","host_type":"Europe_PMC"},{"url":"http://dx.doi.org/10.1007/s00262-021-03101-4","host_type":""},{"url":"https://dx.doi.org/10.1007/s00262-021-03101-4","host_type":""},{"url":"https://hal.science/hal-03428459v1","host_type":""},{"url":"https://hal.science/hal-03428459v1/document","host_type":""},{"url":"https://doi.org/https://doi.org/10.1007/s00262-021-03101-4","host_type":""}],"fields_of_study":["Virus-based gene therapy research","CAR-T cell therapy research","Viral Infectious Diseases and Gene Expression in Insects","0301 basic medicine","0303 health sciences","03 medical and health sciences","Adoptive Transfer","Animals","Disease Models, Animal","Hematopoietic Stem Cell Transplantation","Humans","Leukocytes, Mononuclear","Mice","Mice, SCID"],"mesh_terms":["Animals","Disease Models, Animal","Humans","Leukocytes, Mononuclear","Mice, SCID","Hematopoietic Stem Cell Transplantation","Adoptive Transfer","Mice"],"keywords":["Humanized mouse","Adoptive cell transfer","Reprogramming","Immune system","Immunology","Context (archaeology)","Biology","Transgene","Population","B cell","Antibody","T cell","Medicine","Cell","Gene","Genetics","Gene therapy","Cell therapy","Humanized Immune System","B Cell Adoptive Transfer","Research Report","570","[SDV]Life Sciences [q-bio]","Hematopoietic Stem Cell Transplantation","610","Mice, SCID","Adoptive Transfer","[SDV] Life Sciences [q-bio]","Disease Models, Animal","Mice","Leukocytes, Mononuclear","Animals","Humans"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. Good health"},{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T17:23:21.802802Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}