{"doi":"10.1007/s00253-021-11252-8","title":"Impact of a fermented soy beverage supplemented with acerola by-product on the gut microbiota from lean and obese subjects using an in vitro model of the human colon","abstract":"<jats:sec>\n                <jats:title>Abstract</jats:title>\n                <jats:p>The aim of this study was to evaluate the effects of soy-based beverages manufactured with water-soluble soy extract, containing probiotic strains (<jats:italic>Lactobacillus acidophilus</jats:italic> LA-5 and <jats:italic>Bifidobacterium longum</jats:italic> BB-46) and/or acerola by-product (ABP) on pooled faecal microbiota obtained from lean and obese donors. Four fermented soy beverages (FSs) (“placebo” (FS-Pla), probiotic (FS-Pro), prebiotic (FS-Pre), and synbiotic (FS-Syn)) were subjected to <jats:italic>in vitro</jats:italic> digestion, followed by inoculation in the TIM-2 system, a dynamic <jats:italic>in vitro</jats:italic> model that mimics the conditions of the human colon. Short- and branched-chain fatty acids (SCFA and BCFA) and microbiota composition were determined. Upon colonic fermentation in the presence of the different FSs formulations, acetic and lactic acid production was higher than the control treatment for faecal microbiota from lean individuals (FMLI). Additionally, SCFA production by the FMLI was higher than for the faecal microbiota from obese individuals (FMOI). <jats:italic>Bifidobacterium</jats:italic> spp. and <jats:italic>Lactobacillus</jats:italic> spp. populations increased during simulated colonic fermentation in the presence of FS-Syn in the FMLI and FMOI. FS formulations also changed the composition of the FMOI, resulting in a profile more similar to the FMLI. The changes in the composition and the increase in SCFA production observed for the FMLI and FMOI during these <jats:italic>in vitro</jats:italic> fermentations suggest a potential modulation effect of these microbiotas by the consumption of functional FSs.</jats:p>\n              </jats:sec><jats:sec>\n                <jats:title>Graphical abstract</jats:title>\n                \n              </jats:sec><jats:sec>\n                <jats:title>Key points</jats:title>\n                <jats:p>• <jats:italic>Soy beverages increased Bifidobacterium abundance in microbiota from obese individuals.</jats:italic></jats:p>\n                <jats:p>• <jats:italic>The synbiotic beverage increased Bifidobacterium abundance in microbiota from lean individuals.</jats:italic></jats:p>\n                <jats:p>• <jats:italic>The synbiotic beverage changed the microbiota from obese individuals, approaching the lean profiles.</jats:italic></jats:p>\n              </jats:sec>","journal":"Applied Microbiology and Biotechnology","year":2021,"id":594338,"datarank":0.5495342469194471,"base_score":3.6635616461296463,"endowment":3.6635616461296463,"self_citation_contribution":0.5495342469194471,"citation_network_contribution":0.0,"self_endowment_contribution":0.5495342469194471,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":38,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1521387,"name":"Carlota Bussolo de Souza","orcid":"0000-0001-6663-1962","position":1,"is_corresponding":false},{"id":1521390,"name":"Marina Padilha","orcid":"0000-0002-1736-0411","position":2,"is_corresponding":false},{"id":599918,"name":"Erwin G. Zoetendal","orcid":"0000-0002-7149-0727","position":3,"is_corresponding":false},{"id":772502,"name":"Hauke Smidt","orcid":"0000-0002-6138-5026","position":4,"is_corresponding":false},{"id":255751,"name":"Susana Marta Isay Saad","orcid":"0000-0001-9682-8491","position":5,"is_corresponding":false},{"id":1521393,"name":"Koen Venema","orcid":"0000-0001-7046-5127","position":6,"is_corresponding":false},{"id":1521385,"name":"Antonio Diogo Silva Vieira","orcid":"0000-0003-0484-4369","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Impact of a fermented soy beverage supplemented with acerola by-product on the gut microbiota from lean and obese subjects using an in vitro model of the human colon.","abstract":"The aim of this study was to evaluate the effects of soy-based beverages manufactured with water-soluble soy extract, containing probiotic strains (Lactobacillus acidophilus LA-5 and Bifidobacterium longum BB-46) and/or acerola by-product (ABP) on pooled faecal microbiota obtained from lean and obese donors. Four fermented soy beverages (FSs) (\"placebo\" (FS-Pla), probiotic (FS-Pro), prebiotic (FS-Pre), and synbiotic (FS-Syn)) were subjected to in vitro digestion, followed by inoculation in the TIM-2 system, a dynamic in vitro model that mimics the conditions of the human colon. Short- and branched-chain fatty acids (SCFA and BCFA) and microbiota composition were determined. Upon colonic fermentation in the presence of the different FSs formulations, acetic and lactic acid production was higher than the control treatment for faecal microbiota from lean individuals (FMLI). Additionally, SCFA production by the FMLI was higher than for the faecal microbiota from obese individuals (FMOI). Bifidobacterium spp. and Lactobacillus spp. populations increased during simulated colonic fermentation in the presence of FS-Syn in the FMLI and FMOI. FS formulations also changed the composition of the FMOI, resulting in a profile more similar to the FMLI. The changes in the composition and the increase in SCFA production observed for the FMLI and FMOI during these in vitro fermentations suggest a potential modulation effect of these microbiotas by the consumption of functional FSs. KEY POINTS: • Soy beverages increased Bifidobacterium abundance in microbiota from obese individuals. • The synbiotic beverage increased Bifidobacterium abundance in microbiota from lean individuals. • The synbiotic beverage changed the microbiota from obese individuals, approaching the lean profiles.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"33937924","pmcid":"PMC8102275","openalex_id":null,"authors":[],"funders":[{"funder_name":"Coordenação de Aperfeiçoamento de Pessoal de Nível Superior","grant_id":"1575592","title":null},{"funder_name":"Fundação de Amparo à Pesquisa do Estado de São Paulo","grant_id":"2016/07940-7","title":null},{"funder_name":"Centre for Healthy Eating &amp; Food Innovation","grant_id":"HEFI","title":null},{"funder_name":"Fundação de Amparo à Pesquisa do Estado de São Paulo","grant_id":"2013/19346-4","title":null},{"funder_name":"Conselho Nacional de Desenvolvimento Científico e Tecnológico","grant_id":"306330/2016-4","title":null},{"funder_name":"Fundação de Amparo à Pesquisa do Estado de São Paulo","grant_id":"2013/50506-8","title":null},{"funder_name":"Fundação de Amparo à Pesquisa do Estado de São Paulo","grant_id":"2013/26435-3","title":null},{"funder_name":"Dutch Province of Limburg","grant_id":"HEFI-1","title":null},{"funder_name":"Conselho Nacional de Desenvolvimento Científico e Tecnológico","grant_id":"246027/2012-6","title":null},{"funder_name":"Fundação para a Ciência e a Tecnologia, I.P.","grant_id":"Incentivo/SAU/LA0005/2013","title":"Incentive - LA 5 - 2013"}],"total_grants":10,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by","oa_locations":[{"url":"https://link.springer.com/content/pdf/10.1007/s00253-021-11252-8.pdf","host_type":"publisher"},{"url":"https://cris.maastrichtuniversity.nl/en/publications/486d6bb3-fdb9-433f-86f8-136bd38ecb50","host_type":"repository"},{"url":"https://dx.doi.org/10.1007/s00253-021-11252-8","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/8102275","host_type":"repository"},{"url":"https://research.wur.nl/en/publications/impact-of-a-fermented-soy-beverage-supplemented-with-acerola-by-p","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC8102275","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC8102275?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.1007/s00253-021-11252-8","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/33937924","host_type":""},{"url":"http://dx.doi.org/10.1007/s00253-021-11252-8","host_type":""}],"fields_of_study":["0301 basic medicine","03 medical and health sciences","0303 health sciences"],"mesh_terms":["Colon","Feces","Humans","Obesity","Fermentation","Beverages","Soy Milk","Probiotics","Gastrointestinal Microbiome"],"keywords":["Obesity","Probiotic","in vitro model","Gut Microbiota","Fruit By-product","Colon","CONSENSUS STATEMENT","SUBSP LACTIS GCL2505","Beverages","Feces","Environmental Biotechnology","Humans","LACTOBACILLUS-ACIDOPHILUS","INTERNATIONAL SCIENTIFIC ASSOCIATION","GENE-EXPRESSION","BIFIDOBACTERIUM-ANIMALIS","Probiotics","PROBIOTIC YOGURT","WEIGHT-GAIN","Gastrointestinal Microbiome","Soy Milk","BODY-MASS INDEX","CHAIN FATTY-ACIDS","Fermentation"],"sdg_mappings":[{"sdg_number":2,"sdg_label":"2. 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