{"doi":"10.1007/s00216-010-4117-6","title":"Hyphenated techniques for the analysis of heparin and heparan sulfate","abstract":null,"journal":"Analytical and Bioanalytical Chemistry","year":2011,"id":603538,"datarank":0.6261580904843456,"base_score":4.174387269895637,"endowment":4.174387269895637,"self_citation_contribution":0.6261580904843456,"citation_network_contribution":0.0,"self_endowment_contribution":0.6261580904843456,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":64,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":694183,"name":"Kemal Solakyildirim","orcid":"0000-0002-2241-5544","position":1,"is_corresponding":false},{"id":1548224,"name":"Yuqing Chang","orcid":null,"position":2,"is_corresponding":false},{"id":286697,"name":"Robert J. Linhardt","orcid":"0000-0003-2219-5833","position":3,"is_corresponding":false},{"id":1345346,"name":"Bo Yang","orcid":"0000-0003-4608-6957","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Hyphenated techniques for the analysis of heparin and heparan sulfate","abstract":"The elucidation of the structure of glycosaminoglycan has proven to be challenging for analytical chemists. Molecules of glycosaminoglycan have a high negative charge and are polydisperse and microheterogeneous, thus requiring the application of multiple analytical techniques and methods. Heparin and heparan sulfate are the most structurally complex of the glycosaminoglycans and are widely distributed in nature. They play critical roles in physiological and pathophysiological processes through their interaction with heparin-binding proteins. Moreover, heparin and low-molecular weight heparin are currently used as pharmaceutical drugs to control blood coagulation. In 2008, the health crisis resulting from the contamination of pharmaceutical heparin led to considerable attention regarding their analysis and structural characterization. Modern analytical techniques, including high-performance liquid chromatography, capillary electrophoresis, mass spectrometry, and nuclear magnetic resonance spectroscopy, played critical roles in this effort. A successful combination of separation and spectral techniques will clearly provide a critical advantage in the future analysis of heparin and heparan sulfate. This review focuses on recent efforts to develop hyphenated techniques for the analysis of heparin and heparan sulfate.","is_dataset_classified":null,"base_score":4.174387269895637,"endowment":4.174387269895637,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"20853165","pmcid":"PMC3235348","openalex_id":"https://openalex.org/W2063238557","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"GM38060","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM090257","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"HL101721","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"RC2 HL101721","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM090257","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM038060","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"HL096972","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"R01 HL096972","title":null}],"total_grants":8,"fwci":2.5958,"citation_percentile":0.89099452,"influential_citations":0,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":5},{"year":2014,"count":3},{"year":2015,"count":7},{"year":2016,"count":4},{"year":2017,"count":4},{"year":2018,"count":1},{"year":2019,"count":4},{"year":2020,"count":4},{"year":2021,"count":5},{"year":2022,"count":2},{"year":2023,"count":4},{"year":2024,"count":3},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"green","license":"http://www.springer.com/tdm","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3235348","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3235348","host_type":"repository"},{"url":"http://link.springer.com/content/pdf/10.1007/s00216-010-4117-6.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/s00216-010-4117-6/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/s00216-010-4117-6","host_type":"publisher"},{"url":"https://doi.org/10.1007/s00216-010-4117-6","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/20853165","host_type":"repository"}],"fields_of_study":["Proteoglycans and glycosaminoglycans research","Glycosylation and Glycoproteins Research","Mass Spectrometry Techniques and Applications","Animals","Anticoagulants","Carbohydrate Sequence","Chromatography, High Pressure Liquid","Electrophoresis, Capillary","Heparin","Heparan Sulfate","Humans","Magnetic Resonance Spectroscopy","Molecular Sequence Data","Tandem Mass Spectrometry"],"mesh_terms":["Animals","Anticoagulants","Carbohydrate Sequence","Chromatography, High Pressure Liquid","Heparin","Heparitin Sulfate","Heparan Sulfate","Humans","Molecular Sequence Data","Magnetic Resonance Spectroscopy","Electrophoresis, Capillary","Tandem Mass Spectrometry"],"keywords":["Heparin","Heparan sulfate","Glycosaminoglycan","Chemistry","Capillary electrophoresis","Chromatography","Biochemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"pdb"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T22:11:18.182674Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}