{"doi":"10.1007/s00204-025-04129-6","title":"Zebrafish exposed to a cocktail of pesticides during early development display long-lasting neurobehavioral alterations","abstract":"The widespread use of pesticides is increasing the presence of environmental contaminants with potential impacts on biodiversity, ecosystems, and human health. Although long-term pesticide effects have been previously studied, the long-term impact of an acute pesticide exposure during critical early developmental periods remains poorly understood. Here, we used zebrafish to examine whether acute exposure to a pesticide mixture at 0.5 μg/L (the maximum allowed in drinking water) during the first 5 days post-fertilisation (dpf) of development has lasting effects at 28 dpf. Zebrafish were assessed behaviourally, morphologically, and molecularly both immediately after exposure at 5 dpf and later at 28 dpf. Our results show alterations in stress-response that start to emerge right after the developmental exposure and are associated with a less anxious-like phenotype at juvenile stages. Interestingly, despite the observed behavioural phenotype at 28 dpf, it did not lead to significant molecular changes in the hypothalamic-pituitary-interrenal (HPI) axis at this stage. On the contrary, a positive control group of juvenile fish subjected to a sustained pesticide exposure throughout the 28 dpf showed both reduced anxiety-like behaviour and HPI alterations. Our study suggests that even an acute exposure to a low-concentration of pesticides during critical developmental periods can result in enduring behavioural changes.","journal":"Archives of Toxicology","year":2025,"id":553972,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9518,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1450851,"name":"Isabel Forner-Piquer","orcid":"0000-0002-5315-3858","position":1,"is_corresponding":false},{"id":1450852,"name":"Ieremias Chousidis","orcid":"0000-0001-6356-6034","position":2,"is_corresponding":false},{"id":1451302,"name":"Elliott Godden","orcid":null,"position":3,"is_corresponding":false},{"id":1451303,"name":"Alba García-Deante","orcid":null,"position":4,"is_corresponding":false},{"id":407421,"name":"Nicola Marchi","orcid":"0000-0001-9124-0226","position":5,"is_corresponding":false},{"id":361630,"name":"Caroline H. Brennan","orcid":"0000-0002-4169-4083","position":6,"is_corresponding":false},{"id":898428,"name":"Jose Vicente Torres-Pérez","orcid":"0000-0001-6390-9690","position":7,"is_corresponding":false},{"id":1450850,"name":"María Abellán‐Álvaro","orcid":"0000-0001-8974-330X","position":0,"is_corresponding":true}],"reference_count":49,"raw_metadata":null,"created_at":"2026-07-19T02:54:45.872391Z","pmid":"40853368","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}