{"doi":"10.1007/bf03346813","title":"Chronic exposure to glibenclamide impairs insulin secretion in isolated rat pancreatic islets","abstract":null,"journal":"Journal of Endocrinological Investigation","year":1991,"id":683938,"datarank":0.4493598410330987,"base_score":2.995732273553991,"endowment":2.995732273553991,"self_citation_contribution":0.4493598410330987,"citation_network_contribution":0.0,"self_endowment_contribution":0.4493598410330987,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1786740,"name":"A. M. Rabuazzo","orcid":null,"position":1,"is_corresponding":false},{"id":1786741,"name":"M. Vetri","orcid":null,"position":2,"is_corresponding":false},{"id":1786742,"name":"C. Gatta","orcid":null,"position":3,"is_corresponding":false},{"id":1786743,"name":"C. Vinci","orcid":null,"position":4,"is_corresponding":false},{"id":1786744,"name":"M. Buscema","orcid":null,"position":5,"is_corresponding":false},{"id":1786745,"name":"R. Vigneri","orcid":null,"position":6,"is_corresponding":false},{"id":1786746,"name":"F. Purrello","orcid":null,"position":7,"is_corresponding":false},{"id":1786739,"name":"Damiano Gullo","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Chronic exposure to glibenclamide impairs insulin secretion in isolated rat pancreatic islets","abstract":"We investigated the effect of 24 h exposure to 100 nmol/l glibenclamide on insulin secretion in isolated rat pancreatic islets. The insulin content was similar in control islets and in islets preincubated with 100 nmol/l glibenclamide for 24 h. In islets preexposed to glibenclamide: 1) the subsequent response to a maximal glibenclamide stimulatory concentration (10 mumol/l, 1 h at 37 C) was greatly reduced in comparison to control islets (0.69 +/- 0.20% vs 2.16 +/- 0.41%; mean +/- SE; n = 14; p less than 0.001); 2) the response to 100 mumol/l tolbutamide stimulation was also reduced (0.55 +/- 0.15% vs 2.38 +/- 0.44%; n = 8; p less than 0.001); 3) the response to 16.7 mmo/l glucose, both in the presence or in the absence of 1 mmol/l IBMX, a phosphodiesterase inhibitor, was also diminished by about 50% (1.79 +/- 0.39% vs. 3.22 +/- 0.42%; n = 14, p less than 0.001). In glibenclamide pretreated islets, blunted responses to stimuli were confirmed also by dynamic studies using a perifusion system. The effect of glibenclamide preincubation was fully reversible: when islets cultured in the presence of glibenclamide were transferred to a glibenclamide-free medium for further 24 h, insulin release in response to glibenclamide stimulation returned to control values. We conclude that prolonged exposure of rat pancreatic islets to glibenclamide induces a reversible desensitization to a variety of metabolic stimuli. The inhibition by prolonged glibenclamide exposure of a common pathway in the mechanism of insulin release is one possible explanation for these results.","is_dataset_classified":null,"base_score":2.995732273553991,"endowment":2.995732273553991,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"1907623","pmcid":null,"openalex_id":"https://openalex.org/W2095114253","authors":[],"funders":[],"total_grants":0,"fwci":0.4676,"citation_percentile":0.66485223,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2022,"count":2}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/BF03346813.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/BF03346813/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/BF03346813","host_type":"publisher"},{"url":"https://doi.org/10.1007/bf03346813","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/1907623","host_type":"repository"}],"fields_of_study":["Pancreatic function and diabetes","Diabetes Management and Research","Diabetes Treatment and Management","Animals","Glyburide","In Vitro Techniques","Insulin","Insulin Secretion","Islets of Langerhans","Male","Rats","Rats, Inbred Strains","Time Factors","Tolbutamide"],"mesh_terms":["Insulin Secretion","Animals","Glyburide","Insulin","Islets of Langerhans","Male","Rats, Inbred Strains","Time Factors","Tolbutamide","Rats","In Vitro Techniques"],"keywords":["Glibenclamide","Endocrinology","Internal medicine","Pancreatic islets","Insulin","Tolbutamide","IBMX","Stimulation","Islet","Phosphodiesterase inhibitor","Chemistry","Diabetes mellitus","Medicine","Forskolin"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T12:47:04.576509Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}