{"doi":"10.1007/bf02535262","title":"Effect of dietary<i>trans</i> fatty acids on some membrane‐associated enzymes and receptors in rat heart","abstract":"<jats:title>Abstract</jats:title><jats:p>Three groups of male weanling Sprague‐Dawley rats were fed diets containing 20% corn oil, 20% partially hydrogenated soybean oil (PHSBO) or 18% PHSBO +2% corn oil. PHSBO contained about 48% of its total fatty acids as<jats:italic>trans</jats:italic>‐octadecenoate. Rats were killed after 16–18 weeks of feeding the various diets, hearts were dissected and crude sarcolemma was prepared by differential centrifugation. The activities of ouabain‐sensitive (Na<jats:sup>+</jats:sup>+K<jats:sup>+</jats:sup>)ATPase were significantly lower in membranes of rats fed 20% PHSBO than the control rats fed 20% corn oil. The feeding of 2% corn oil with 18% PHSBO resulted in partial restoration of the enzyme activity. The maximum number of [<jats:sup>3</jats:sup>H]ouabain‐binding sites (B<jats:sub>max</jats:sub>) was also lower in cardiac membranes of rats fed 20% PHSBO than those fed 20% corn oil. Similar to (Na<jats:sup>+</jats:sup>+K<jats:sup>+</jats:sup>)ATPase activity, some restoration of the number of [<jats:sup>3</jats:sup>H]ouabain‐binding sites was observed when 2% corn oil was fed with 18% PHSBO‐containing diet. There was no difference in the binding affinity of the radioligand for the receptor among the 3 dietary groups. Adenylate cyclase activities (fluoride‐, isoproterenol‐ and forskolinstimulated) were lower in membranes of rats fed 20% PHSBO or 18% PHSBO +2% corn oil than in the control group fed 20% corn oil. Density of the β‐adrenergic receptor was the lowest in cardiac membranes of rats fed 20% PHSBO. The feeding of 2% corn oil with 18% PHSBO resulted in partial restoration of the maximum number of [<jats:sup>3</jats:sup>H]dihydroalprenolol (DHA)‐binding sites. The affinity of the binding sites was, however, not affected by the type of the dietary fat. The results of this study suggest that dietary<jats:italic>trans</jats:italic> fatty acids can affect the activities of (Na<jats:sup>+</jats:sup>+K<jats:sup>+</jats:sup>)ATPase and adenylate cyclase and the density of digitalis and β‐adrenergic receptors in rat heart.</jats:p>","journal":"Lipids","year":1989,"id":20227,"datarank":0.5101796072493234,"base_score":3.4011973816621555,"endowment":3.4011973816621555,"self_citation_contribution":0.5101796072493234,"citation_network_contribution":0.0,"self_endowment_contribution":0.5101796072493234,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":29,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":134451,"name":"Yun‐Fang Ren","orcid":null,"position":1,"is_corresponding":false},{"id":134452,"name":"Bassima S. Alam","orcid":null,"position":2,"is_corresponding":false},{"id":134450,"name":"Syed Q. Alam","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2545996","pmcid":null,"openalex_id":"https://openalex.org/W2545996","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.00021889,"influential_citations":1,"citation_trend":[],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://aocs.onlinelibrary.wiley.com/doi/pdf/10.1007/BF02535262","host_type":"publisher"},{"url":"https://cyberleninka.ru/article/n/statisticheskaya-otsenka-vesovogo-koeffitsienta-v-kombinirovannoy-modeli-regressii.pdf","host_type":"journal"}],"fields_of_study":["Advanced Research in Systems and Signal Processing","Aerospace, Electronics, Mathematical Modeling","Engineering Diagnostics and Reliability","Biology","Medicine","Agricultural and Food Sciences","Adenylyl Cyclases","Animals","Binding Sites","Cell Membrane","Dietary Fats","Fatty Acids","Heart","Male","Membrane Lipids","Myocardium","Rats","Rats, Inbred Strains","Receptors, Adrenergic, beta","Sodium-Potassium-Exchanging ATPase"],"mesh_terms":["Myocardium","Heart","Cell Membrane","Animals","Rats, Inbred Strains","Rats","Dietary Fats","Fatty Acids","Membrane Lipids","Receptors, Adrenergic, beta","Binding Sites","Male","Sodium-Potassium-Exchanging ATPase","Adenylyl Cyclases"],"keywords":["Weighting","Statistics","Parametric statistics","Regression analysis","Regression","Mathematics","Statistical model","Nonparametric regression","Semiparametric regression","Linear regression","Standard deviation","Computer science"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Reduced inequalities"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-04T07:49:32.319726Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}