{"doi":"10.1007/bf02522925","title":"Studies on phospholipid biosynthesis in hepatocytes from alcoholic rats by using radiolabeled exogenous precursors","abstract":"<jats:title>Abstract</jats:title><jats:p>We have studied the synthesis of phospholipids in hepatocytes isolated from chronically ethanol‐treated rats by using isotopically labelled serine, ethanolamine, and choline as exogenous precursors. Our results demonstrate that ethanol induces specific effects on the biosynthesis of phosphatidyl‐ethanolamine and phosphatidylcholine<jats:italic>via</jats:italic> CDP‐derivatives and also on the synthesis of phosphatidylserine<jats:italic>via</jats:italic> the Ca<jats:sup>++</jats:sup>‐dependent base‐exchange reaction. Thus, the synthesis of phosphatidylethanolamine from [<jats:sup>3</jats:sup>‐H]ethanolamine and the incorporation of [<jats:sup>3</jats:sup>H]serine into phosphatidylserine were clearly higher in hepatocytes from ethanol‐treated rats compared to controls. The synthesis of phosphatidylcholine from [methyl‐<jats:sup>14</jats:sup>C] choline, on the other hand, decreased markedly, suggesting a specific inhibition of cholinephosphotransferase activity. We have also demonstrated that the phosphatidylcholine levels are markedly decreased in hepatocytes isolated from chronically ethanol‐treated rats as a consequence of the lower phosphatidylcholine biosynthesis. The decrease in the incorporation of radioactivity from choline to betaine, which we also found, is interpreted as being the result of a higher use of betaine as methyl donor instead of methionine to maintain the hepatic<jats:italic>S</jats:italic>‐adenosylmethionine levels in chronic alcoholism.</jats:p>","journal":"Lipids","year":1996,"id":35079,"datarank":0.8181674301570281,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"self_citation_contribution":0.44166584687496613,"citation_network_contribution":0.376501583282062,"self_endowment_contribution":0.44166584687496613,"citer_contribution":0.376501583282062,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":8,"citers_with_citation_signal":6,"citers_with_endowment":6,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":180421,"name":"M. C. Sanchez‐Amate","orcid":null,"position":1,"is_corresponding":false},{"id":180422,"name":"J. L. Segovia","orcid":null,"position":2,"is_corresponding":false},{"id":172475,"name":"C. Marco","orcid":null,"position":3,"is_corresponding":false},{"id":180420,"name":"M. P. Carrasco","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.9444389791664403,"endowment":2.9444389791664403,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8743051","pmcid":null,"openalex_id":"https://openalex.org/W2031498465","authors":[],"funders":[],"total_grants":0,"fwci":2.1994,"citation_percentile":0.86961793,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"http://onlinelibrary.wiley.com/termsAndConditions#vor","oa_locations":[{"url":"https://aocs.onlinelibrary.wiley.com/doi/pdf/10.1007/BF02522925","host_type":"publisher"},{"url":"https://doi.org/10.1007/bf02522925","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8743051","host_type":"repository"}],"fields_of_study":["Liver Disease Diagnosis and Treatment","Alcohol Consumption and Health Effects","Diet, Metabolism, and Disease","Chemistry","Medicine","Biology","Alcoholism","Animals","Carbon Radioisotopes","Cells, Cultured","Choline","Diacylglycerol Cholinephosphotransferase","Ethanolamine","Ethanolamines","Liver","Male","Phosphatidylcholines","Phosphatidylethanolamines","Phosphatidylserines","Phospholipids","Radioisotope Dilution Technique","Rats","Rats, Sprague-Dawley","Reference Values","Serine","Tritium"],"mesh_terms":["Alcoholism","Animals","Carbon Radioisotopes","Cells, Cultured","Choline","Diacylglycerol Cholinephosphotransferase","Ethanolamines","Liver","Male","Phosphatidylcholines","Phosphatidylethanolamines","Phosphatidylserines","Phospholipids","Radioisotope Dilution Technique","Reference Values","Serine","Tritium","Rats, Sprague-Dawley","Ethanolamine","Rats"],"keywords":["Phosphatidylethanolamine","Phosphatidylserine","Phosphatidylcholine","Ethanolamine","Choline","Betaine","Phospholipid","Biosynthesis","Biochemistry","Chemistry","Serine","Methionine","Phosphocholine","Ethanol","Amino acid","Enzyme"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-10T00:40:07.123618Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}