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Lipids form an essential part of the N-glycosylation pathway, and have been shown to improve cell viability. In control (serum-free) medium the proportion of fully-glycosylated interferon-gamma deteriorated reproducibly with time in batch culture, but the lipoprotein supplement ExCyte was shown to minimise this trend. Partially substituting the bovine serum albumin content of the medium with a fatty-acid free preparation also improved interferon-gamma glycosylation, possibly indicating that oxidised lipids carried on Cohn fraction V albumin may damage the glycosylation process.","is_dataset_classified":null,"base_score":4.07753744390572,"endowment":4.07753744390572,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"7765933","pmcid":null,"openalex_id":"https://openalex.org/W2014664217","authors":[],"funders":[],"total_grants":0,"fwci":1.1936,"citation_percentile":0.77631775,"influential_citations":6,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":6},{"year":2014,"count":1},{"year":2015,"count":3},{"year":2017,"count":2},{"year":2018,"count":1},{"year":2020,"count":2},{"year":2021,"count":1},{"year":2022,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/BF00762395.pdf","host_type":"publisher"},{"url":"http://link.springer.com/article/10.1007/BF00762395/fulltext.html","host_type":"publisher"},{"url":"http://link.springer.com/content/pdf/10.1007/BF00762395","host_type":"publisher"},{"url":"https://doi.org/10.1007/bf00762395","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/7765933","host_type":"repository"},{"url":"https://kar.kent.ac.uk/20272/","host_type":"repository"}],"fields_of_study":["Viral Infectious Diseases and Gene Expression in Insects","Glycosylation and Glycoproteins Research","Monoclonal and Polyclonal Antibodies Research","Biology","Medicine","Chemistry","Animals","CHO Cells","Cell Count","Cell Division","Cell Survival","Cholesterol","Cricetinae","Culture Media","Culture Techniques","Fatty Acids, Nonesterified","Fatty Acids, Unsaturated","Glycosylation","Humans","Interferon-gamma","Kinetics","Lipids","Phospholipids","Recombinant Proteins","Time Factors"],"mesh_terms":["Animals","Cell Count","Cell Division","Cell Survival","Cholesterol","Culture Media","Fatty Acids, Nonesterified","Fatty Acids, Unsaturated","Glycosylation","Cricetinae","Humans","Interferon-gamma","Kinetics","Lipids","Phospholipids","Recombinant Proteins","Time Factors","CHO Cells","Culture Techniques"],"keywords":["Recombinant DNA","Glycosylation","Chinese hamster ovary cell","Interferon","Biology","Cell culture","Interferon γ","Virology","Interferon gamma","Chemistry","In vitro","Biochemistry","Gene","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T12:40:34.556224Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}