{"doi":"10.1007/978-1-62703-218-6_35","title":"Antigen Processing for MHC Presentation via Macroautophagy","abstract":null,"journal":"Methods in Molecular Biology","year":2013,"id":629774,"datarank":0.3596842909197557,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.0,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1524894,"name":"Rosa Barreira da Silva","orcid":null,"position":1,"is_corresponding":false},{"id":263547,"name":"Christian Münz","orcid":"0000-0001-6419-1940","position":2,"is_corresponding":false},{"id":1524893,"name":"Monique Gannage","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Antigen Processing for MHC Presentation via Macroautophagy","abstract":"Macroautophagy has recently emerged as an important catabolic process involved not only in innate immunity but also in adaptive immunity. Initially described to deliver intracellular antigens to MHC class II loading compartments, its molecular machinery has now also been described to enhance the delivery of extracellular antigens to MHC class II loading compartments by accelerating phagosome maturation. Therefore in pathological situations (viral or bacterial infections, tumorigenesis) the pathway might be involved in shaping CD4(+) T cell responses.In this chapter we describe three basic experiments for the monitoring and manipulation of macroautophagic antigen processing towards MHC class II presentation. Firstly, we will discuss how to monitor autophagic flux and autophagosome fusion with MHC class II loading compartments. Secondly, we will show how to target proteins to autophagosomes in order to monitor macroautophagy-dependent antigen processing via their enhanced presentation on MHC class II molecules to CD4(+) T cells. And finally, we will describe how macroautophagy can be silenced in antigen presenting cells, like human monocyte-derived dendritic cells (DCs).","is_dataset_classified":null,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23329508","pmcid":null,"openalex_id":"https://openalex.org/W165556355","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"R01CA108609","title":null},{"funder_name":"Swiss National Science Foundation","grant_id":"143979","title":"Mechanisms and functional relevance of macroautophagy inhibition during influenza A virus infection"}],"total_grants":2,"fwci":7.3173,"citation_percentile":0.96737235,"influential_citations":0,"citation_trend":[{"year":2013,"count":1},{"year":2014,"count":4},{"year":2015,"count":1},{"year":2016,"count":2},{"year":2021,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"Springer TDM","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-62703-218-6_35","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-62703-218-6_35","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23329508","host_type":"repository"},{"url":"https://iris.unil.ch/handle/iris/244320","host_type":"repository"},{"url":"https://serval.unil.ch/notice/serval:BIB_FF3B4FF6D578","host_type":"repository"},{"url":"https://dx.doi.org/10.5167/uzh-90076","host_type":""},{"url":"https://dx.doi.org/10.1007/978-1-62703-218-6_35","host_type":""},{"url":"https://sonar.ch/global/documents/236262","host_type":""},{"url":"https://www.zora.uzh.ch/id/eprint/90076/","host_type":""},{"url":"https://doi.org/10.5167/uzh-90076","host_type":""},{"url":"http://dx.doi.org/10.1007/978-1-62703-218-6_35","host_type":""}],"fields_of_study":["Autophagy in Disease and Therapy","HIV Research and Treatment","Mosquito-borne diseases and control","0301 basic medicine","03 medical and health sciences","0303 health sciences"],"mesh_terms":["Histocompatibility Antigens Class II","Antigens, Neoplasm","Autophagy","Clone Cells","Dendritic Cells","Enzyme-Linked Immunosorbent Assay","Humans","Immunohistochemistry","Interferon-gamma","Membrane Proteins","Microtubule-Associated Proteins","Monocytes","Recombinant Fusion Proteins","Transfection","Antigen Presentation","Microscopy, Confocal","HEK293 Cells"],"keywords":["Antigen processing","MHC class I","Antigen presentation","MHC class II","Cell biology","Biology","Phagosome","Transporter associated with antigen processing","Antigen","Acquired immune system","Cross-presentation","MHC restriction","CD74","Autophagy","Major histocompatibility complex","T cell","Immunology","Intracellular","Immune system","Genetics","Recombinant Fusion Proteins","610 Medicine & health","Enzyme-Linked Immunosorbent Assay","10263 Institute of Experimental Immunology","Transfection","Monocytes","Interferon-gamma","1311 Genetics","Antigens, Neoplasm","1312 Molecular Biology","Humans","610 Medicine & health","Microscopy, Confocal","Histocompatibility Antigens Class II","Membrane Proteins","Dendritic Cells","Immunohistochemistry","Clone Cells","HEK293 Cells","570 Life sciences; biology","Microtubule-Associated Proteins"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T19:38:04.311710Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}