{"doi":"10.1007/978-1-61779-533-6_1","title":"Regulation of Cre Recombinase: Use of Ligand-Regulated and Dimerizable Cre for Transgenesis","abstract":null,"journal":"Neuromethods","year":2012,"id":625543,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":478751,"name":"Nicolas Jullien","orcid":"0000-0001-8273-2106","position":1,"is_corresponding":false},{"id":1617830,"name":"Jean-Paul Herman","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Regulation of Cre Recombinase: Use of Ligand-Regulated and Dimerizable Cre for Transgenesis","abstract":"Drug-regulatable site-specific recombinases have become, during the last decade, a central tool for ­transgenesis. They allow, depending on the constructs used, the activation or inactivation of target genes in a time-dependent or, if combined with the use of a specific promoter, in a time- and cell-type-dependent manner. The most widely used approach to obtain this regulation relies on the fusion of the recombinase, mostly Cre recombinase, with the ligand-binding domain (LBD) of a steroid receptor, with the resulting Cre-LBD molecule regulated by the cognate steroid. Another, still experimental, method is based on the dimerization of two complementing inactive fragments of Cre, with the drug-induced or spontaneous association of the fragments leading to a reinstatement of the recombinase activity. These different approaches will be described, with a special emphasis on the practical aspects: choice and efficacy of the different variants, drug regimens, and potential pitfalls.","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19910364","pmcid":null,"openalex_id":"https://openalex.org/W2480329044","authors":[],"funders":[],"total_grants":0,"fwci":0.4878,"citation_percentile":0.70039397,"influential_citations":0,"citation_trend":[{"year":2013,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-61779-533-6_1","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-61779-533-6_1","host_type":"book series"}],"fields_of_study":["Pluripotent Stem Cells Research","RNA Interference and Gene Delivery","CRISPR and Genetic Engineering"],"mesh_terms":[],"keywords":["Recombinase","Transgenesis","Cre recombinase","Computational biology","Biology","Transgene","Cell biology","Genetics","Gene","Genetically modified mouse","Recombination"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T07:03:28.614668Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}