{"doi":"10.1007/978-1-61779-270-0_29","title":"Small-Molecule Collection and High-Throughput Colorimetric Assay to Identify PARP1 Inhibitors","abstract":null,"journal":"Methods in Molecular Biology","year":2011,"id":652371,"datarank":0.40620753016533157,"base_score":2.70805020110221,"endowment":2.70805020110221,"self_citation_contribution":0.40620753016533157,"citation_network_contribution":0.0,"self_endowment_contribution":0.40620753016533157,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1701726,"name":"Aaron D. Pinnola","orcid":null,"position":1,"is_corresponding":false},{"id":519630,"name":"Alexei V. Tulin","orcid":"0000-0001-9618-4243","position":2,"is_corresponding":false},{"id":996766,"name":"Elena Kotova","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Small-Molecule Collection and High-Throughput Colorimetric Assay to Identify PARP1 Inhibitors","abstract":"During the last few years, poly(ADP-ribose)polymerase (PARP) proteins became a very popular target for anticancer treatment. Many PARP inhibitors have been generated and tested by pharmacological industry. However, most of them were designed to disrupt the DNA-dependent PARP1 protein activation pathway and were based on a competition with NAD for a binding site on PARP molecule and, therefore, on disruption of PARP-mediated enzymatic reaction. This limitation resulted in a discovery of mainly nucleotide-like PARP1 inhibitors which may target not only PARP, but also other pathways involving NAD and other nucleotides. Here, we describe a strategy for the identification of PARP inhibitors that target a different pathway, the histone H4-dependent PARP1 activation. Besides the identification of NAD competitors in a small-molecule collection, this approach allows finding novel classes of PARP inhibitors that specifically disrupt H4-based PARP activation.","is_dataset_classified":null,"base_score":2.70805020110221,"endowment":2.70805020110221,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21870279","pmcid":"PMC3164787","openalex_id":"https://openalex.org/W26640823","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM077452","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"R01 DK082623","title":null}],"total_grants":2,"fwci":2.4646,"citation_percentile":0.89069649,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2015,"count":1},{"year":2016,"count":2},{"year":2017,"count":2},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-61779-270-0_29","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-61779-270-0_29","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/21870279","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3164787","host_type":"repository"}],"fields_of_study":["PARP inhibition in cancer therapy","Integrated Circuits and Semiconductor Failure Analysis","DNA Repair Mechanisms","Animals","Blotting, Western","Cell Line","Cell Line, Tumor","Colorimetry","Drosophila","Enzyme Inhibitors","Humans","Poly(ADP-ribose) Polymerase Inhibitors"],"mesh_terms":["Poly(ADP-ribose) Polymerase Inhibitors","Animals","Cell Line","Colorimetry","Drosophila","Enzyme Inhibitors","Humans","Blotting, Western","Cell Line, Tumor"],"keywords":["Poly ADP ribose polymerase","NAD+ kinase","PARP1","ADP-ribosylation","Small molecule","Histone","Polymerase","Chemistry","Enzyme","Biochemistry","Biology","Computational biology","DNA","Cell biology"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Industry, innovation and infrastructure"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T13:18:19.868859Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}