{"doi":"10.1007/978-1-60327-090-8_12","title":"Whole-Cell Voltage Clamp on Skeletal Muscle Fibers With the Silicone-Clamp Technique","abstract":null,"journal":"Methods in Molecular Biology™","year":null,"id":676431,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1767410,"name":"Claude Collet","orcid":null,"position":1,"is_corresponding":false},{"id":151804,"name":"Bruno Allard","orcid":null,"position":2,"is_corresponding":false},{"id":988541,"name":"Vincent Jacquemond","orcid":"0000-0003-4944-270X","position":3,"is_corresponding":false},{"id":579755,"name":"Sandrine Pouvreau","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Whole-Cell Voltage Clamp on Skeletal Muscle Fibers With the Silicone-Clamp Technique","abstract":"Control of membrane voltage and membrane current measurements are of strong interest for the study of numerous aspects of skeletal muscle physiology and pathophysiology. The silicone-clamp technique makes use of a conventional patch-clamp apparatus to achieve whole-cell voltage clamp of a restricted portion of a fully differentiated adult skeletal muscle fiber. The major part of an isolated muscle fiber is insulated from the extracellular medium with silicone grease, and the tip of a single microelectrode connected to the amplifier is then inserted within the fiber through the silicone layer. This method represents an alternative to the traditional vaseline-gap isolation and two or three microelectrode voltage-clamp techniques. This chapter reviews the main benefits of the silicone-clamp technique and provides detailed insights into its practical implementation.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21097893","pmcid":null,"openalex_id":"https://openalex.org/W4244325021","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.53859701,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://doi.org/10.1007/978-1-60327-090-8_12","host_type":"ebook platform"},{"url":"https://hal.inrae.fr/hal-02822699","host_type":"repository"}],"fields_of_study":["Muscle activation and electromyography studies","Advanced Sensor and Energy Harvesting Materials","Neuroscience and Neural Engineering"],"mesh_terms":[],"keywords":["Clamp","Voltage clamp","Current clamp","Materials science","Skeletal muscle","Microelectrode","Silicone","Fiber","Biomedical engineering","Anatomy","Chemistry","Biophysics","Electrode","Composite material","Membrane potential","Biology","Medicine","Engineering","Mechanical engineering"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Affordable and clean energy"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T02:32:41.796066Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}