{"doi":"10.1007/978-1-4939-9841-8_8","title":"Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy","abstract":null,"journal":"Methods in Molecular Biology","year":2020,"id":588684,"datarank":0.7344960084904286,"base_score":3.2188758248682006,"endowment":3.2188758248682006,"self_citation_contribution":0.48283137373023016,"citation_network_contribution":0.2516646347601985,"self_endowment_contribution":0.48283137373023016,"citer_contribution":0.2516646347601985,"corpus_percentile":null,"corpus_rank":null,"citation_count":24,"citer_count":12,"citers_with_citation_signal":9,"citers_with_endowment":9,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":618663,"name":"David A. Long","orcid":"0000-0001-6580-3435","position":1,"is_corresponding":false},{"id":1506083,"name":"Peter J. Scambler","orcid":null,"position":2,"is_corresponding":false},{"id":906074,"name":"Dale Moulding","orcid":"0000-0002-1431-7047","position":3,"is_corresponding":false},{"id":409863,"name":"Daniyal J. Jafree","orcid":"0000-0001-8235-0394","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy","abstract":"Microscopic and macroscopic evaluation of biological tissues in three dimensions is becoming increasingly popular. This trend is coincident with the emergence of numerous tissue clearing strategies, and advancements in confocal and two-photon microscopy, enabling the study of intact organs and systems down to cellular and sub-cellular resolution. In this chapter, we describe a wholemount immunofluorescence technique for labeling structures in renal tissue. This technique combined with solvent-based tissue clearing and confocal imaging, with or without two-photon excitation, provides greater structural information than traditional sectioning and staining alone. Given the addition of paraffin embedding to our method, this hybrid protocol offers a powerful approach to combine confocal or two-photon findings with histological and further immunofluorescent analysis within the same tissue.","is_dataset_classified":null,"base_score":3.2188758248682006,"endowment":3.2188758248682006,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31701448","pmcid":null,"openalex_id":"https://openalex.org/W2987417196","authors":[],"funders":[{"funder_name":"Medical Research Council","grant_id":"MR/P018629/1","title":"Investigating the renal microvasculature in polycystic kidney disease"},{"funder_name":"Great Ormond Street Hospital Childrens Charity","grant_id":"V2318","title":null},{"funder_name":"Medical Research Council","grant_id":"MR/J003638/1","title":null},{"funder_name":"British Heart Foundation","grant_id":"RG/15/14/31880","title":null},{"funder_name":"Kidney Research UK","grant_id":"RP36/2015","title":null},{"funder_name":"Kidney Research UK","grant_id":"IN_012_20190306","title":null}],"total_grants":6,"fwci":7.3547,"citation_percentile":0.97184685,"influential_citations":0,"citation_trend":[{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":4},{"year":2022,"count":1},{"year":2023,"count":2},{"year":2024,"count":6},{"year":2025,"count":4},{"year":2026,"count":4}],"oa_status":"closed","license":"Springer TDM","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-4939-9841-8_8","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-4939-9841-8_8","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31701448","host_type":"repository"},{"url":"https://discovery.ucl.ac.uk/id/eprint/10093852/","host_type":"repository"},{"url":"https://discovery.ucl.ac.uk/10093852/3/Long_180710_Compiled%20manuscript%20for%20RPS%20upload.pdf","host_type":""},{"url":"https://dx.doi.org/10.1007/978-1-4939-9841-8_8","host_type":""},{"url":"https://discovery-pp.ucl.ac.uk/id/eprint/10093852/","host_type":""}],"fields_of_study":["Advanced Fluorescence Microscopy Techniques","Advanced Electron Microscopy Techniques and Applications","Cell Image Analysis Techniques","0301 basic medicine","0303 health sciences","03 medical and health sciences","Animals","Histocytological Preparation Techniques","Humans","Imaging, Three-Dimensional","Kidney","Mice","Microscopy, Confocal","Microscopy, Fluorescence, Multiphoton","Software","Solvents","Staining and Labeling","Workflow"],"mesh_terms":["Animals","Humans","Kidney","Software","Solvents","Staining and Labeling","Histocytological Preparation Techniques","Microscopy, Confocal","Imaging, Three-Dimensional","Microscopy, Fluorescence, Multiphoton","Mice","Workflow"],"keywords":["Confocal","Confocal microscopy","Two-photon excitation microscopy","Optical sectioning","Microscopy","Pathology","Biomedical engineering","Optics","Materials science","Medicine","Physics","Fluorescence","Kidney","Imaging","Two-photon","Three-dimensional","Tissue Clearing","Histocytological Preparation Techniques","Microscopy, Confocal","Staining and Labeling","Workflow","Mice","Imaging, Three-Dimensional","Microscopy, Fluorescence, Multiphoton","Solvents","Animals","Humans","Software"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-22T18:57:25.855736Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}