{"doi":"10.1007/978-1-4939-9027-6_22","title":"Overview of Glutamine Dependency and Metabolic Rescue Protocols","abstract":null,"journal":"Methods in Molecular Biology","year":2019,"id":600846,"datarank":0.32958368660043297,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.0,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":562107,"name":"Dan He","orcid":"0000-0002-9544-5672","position":1,"is_corresponding":false},{"id":208925,"name":"Nianli Sang","orcid":null,"position":2,"is_corresponding":false},{"id":506935,"name":"Shuo Qie","orcid":"0000-0002-3481-3582","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Overview of Glutamine Dependency and Metabolic Rescue Protocols","abstract":"Enhanced glutaminolysis and glycolysis are the two most remarkable biochemical features of cancer cell metabolism, reflecting increased utilization of glutamine and glucose in proliferating cells. Most solid tumors often outgrow the blood supply, resulting in a tumor microenvironment characterized by the depletion of glutamine, glucose, and oxygen. Whereas mechanisms by which cancer cells sense and metabolically adapt to hypoxia have been well characterized with a variety of cancer types, mechanisms by which different types of tumor cells respond to a dynamic change of glutamine availability and the underlying importance remains to be characterized. Here we describe the protocol, which uses cultured Hep3B cells as a model in determining glutamine-dependent proliferation, metabolite rescuing, and cellular responses to glutamine depletion. These protocols may be modified to study the metabolic roles of glutamine in other types of tumor or non-tumor cells as well.","is_dataset_classified":null,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30725468","pmcid":null,"openalex_id":"https://openalex.org/W2914007480","authors":[],"funders":[{"funder_name":"NIDCR NIH HHS","grant_id":"T32 DE017551","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA129494","title":null}],"total_grants":2,"fwci":1.568,"citation_percentile":0.83987603,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2021,"count":1},{"year":2022,"count":2},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-4939-9027-6_22","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-4939-9027-6_22","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30725468","host_type":"repository"}],"fields_of_study":["Cancer, Hypoxia, and Metabolism","Cancer Research and Treatments","Cancer-related Molecular Pathways","Adaptation, Biological","Cell Culture Techniques","Cell Line, Tumor","Cell Proliferation","Endoplasmic Reticulum Stress","Energy Metabolism","Gene Expression Regulation, Neoplastic","Glucose","Glutamine","Glycolysis","Humans","Metabolic Networks and Pathways","Neoplasms","Oxygen","Signal Transduction"],"mesh_terms":["Adaptation, Biological","Energy Metabolism","Glucose","Glutamine","Glycolysis","Humans","Neoplasms","Oxygen","Signal Transduction","Gene Expression Regulation, Neoplastic","Cell Culture Techniques","Cell Line, Tumor","Cell Proliferation","Metabolic Networks and Pathways","Endoplasmic Reticulum Stress"],"keywords":["Glutaminolysis","Glutamine","Glycolysis","Tumor microenvironment","Cancer cell","Hypoxia (environmental)","Biology","Metabolism","Biochemistry","Cell biology","Metabolite","Cancer","Chemistry","Cancer research","Tumor cells","Amino acid","Oxygen","Genetics","Cell proliferation","endoplasmic reticulum stress","Anaplerosis","Glutamine Depletion","Nitrogen Anabolism"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T14:18:08.081189Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}