{"doi":"10.1007/978-1-4939-9012-2_18","title":"In Situ Hybridization in Mineralized Tissues: The Added Value of LNA Probes for RNA Detection","abstract":null,"journal":"Methods in Molecular Biology","year":2019,"id":675224,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1764231,"name":"D. Hotton","orcid":null,"position":1,"is_corresponding":false},{"id":1764232,"name":"A. Berdal","orcid":null,"position":2,"is_corresponding":false},{"id":1764233,"name":"A. Bolaños","orcid":null,"position":3,"is_corresponding":false},{"id":1764230,"name":"G. Lignon","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"In Situ Hybridization in Mineralized Tissues: The Added Value of LNA Probes for RNA Detection","abstract":"In situ hybridization (ISH) is one of the fundamental methods in developmental biology and neurobiology. Their first ISH protocols were reported in 1969 (Gall and Pardue, Proc Natl AcadSci USA 63:378-83, 1969). Since several decades, ISH based on the specific hybridization of 100-2000 nucleotides long probes enabled the localization of DNA/RNA sequences in tissues and cells with high cellular resolution. But sometimes a limited sensitivity notably in mineralized tissues (Obernosterer et al., Nature Protocols 2:1508-14, 2007).Here we describe a recent improvement of in situ hybridization efficiency by applying nucleotide locked nucleic acid (LNA)-incorporated oligodeoxynucleotide probes (20 LNA/DNA nucleotide probes) essentially used for noncoding miRNA and messenger RNAs.","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30838576","pmcid":null,"openalex_id":"https://openalex.org/W2920512355","authors":[],"funders":[],"total_grants":0,"fwci":0.3183,"citation_percentile":0.56503559,"influential_citations":0,"citation_trend":[{"year":2019,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-4939-9012-2_18","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-4939-9012-2_18","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30838576","host_type":"repository"}],"fields_of_study":["Molecular Biology Techniques and Applications","RNA regulation and disease","RNA modifications and cancer","Animals","DNA Probes","In Situ Hybridization","Mice","MicroRNAs","Paraffin Embedding","RNA, Messenger","Tooth"],"mesh_terms":["Animals","RNA, Messenger","Tooth","DNA Probes","Paraffin Embedding","In Situ Hybridization","MicroRNAs","Mice"],"keywords":["In situ hybridization","In situ","Value (mathematics)","Chemistry","Materials science","Biochemistry","Computer science","Messenger RNA","Gene","Organic chemistry","Specificity","RNA probe","Dental Mineralized Tissue","Lna/dna Probe"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-16T20:20:44.210437Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}