{"doi":"10.1007/978-1-4939-8754-2_10","title":"Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) During Necroptosis","abstract":null,"journal":"Methods in Molecular Biology","year":2018,"id":685340,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":490524,"name":"Adrian T. Ting","orcid":"0000-0003-0653-9923","position":1,"is_corresponding":false},{"id":490520,"name":"Rosalind L. Ang","orcid":"0000-0001-5836-4243","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) During Necroptosis","abstract":"FAS-associated protein with death domain (FADD) is a signaling molecule required by members of the TNF receptor superfamily (TNFRSF) such as FAS and TNFR1 to induce apoptosis. FADD is a small adapter molecule that functions as a scaffold to recruit procaspase-8 and other regulators. The FADD-containing signaling complex that initiates the apoptotic cascade has been termed the death inducing signaling complex (DISC). In the absence of FADD, death receptors cannot induce apoptosis and in appropriate cell types, these death receptors then induce necroptosis. Necroptosis can also be induced by death receptors in FADD-sufficient cells when caspase-8 is inhibited, usually accomplished by the addition of caspase inhibitors. Under such necroptotic conditions, the immunoprecipitation of FADD to isolate the DISC can be utilized to examine components of this complex. Here, we describe the immunoprecipitation of FADD and subsequent western-blotting to identify RIPK1 in this complex during necroptosis.","is_dataset_classified":null,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30136234","pmcid":null,"openalex_id":"https://openalex.org/W2888494306","authors":[],"funders":[{"funder_name":"NIDDK NIH HHS","grant_id":"P01 DK072201","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI052417","title":null}],"total_grants":2,"fwci":1.1969,"citation_percentile":0.78911565,"influential_citations":0,"citation_trend":[{"year":2019,"count":2},{"year":2020,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-4939-8754-2_10","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-4939-8754-2_10","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30136234","host_type":"repository"}],"fields_of_study":["Cell death mechanisms and regulation","Immune Response and Inflammation","Phagocytosis and Immune Regulation","Animals","Apoptosis","Cells, Cultured","Death Domain Receptor Signaling Adaptor Proteins","Embryo, Mammalian","Fas-Associated Death Domain Protein","Fibroblasts","Mice","Necrosis","Receptor-Interacting Protein Serine-Threonine Kinases","Signal Transduction"],"mesh_terms":["Animals","Cells, Cultured","Embryo, Mammalian","Fibroblasts","Necrosis","Signal Transduction","Apoptosis","Mice","Fas-Associated Death Domain Protein","Death Domain Receptor Signaling Adaptor Proteins","Receptor-Interacting Protein Serine-Threonine Kinases"],"keywords":["FADD","Necroptosis","RIPK1","Death domain","Cell biology","TRADD","Caspase 8","Programmed cell death","Signal transduction","Immunoprecipitation","Biology","Apoptosis","Receptor","Chemistry","Caspase","Immunology","Biochemistry","Antibody","Western blotting","Coimmunoprecipitation","Death Inducing Signaling Complex (Disc)","Fas-associated Protein With Death Domain (Fadd)","Receptor-interacting Protein Kinase 1 (Ripk1)"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T16:23:24.823402Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}