{"doi":"10.1007/978-1-4939-8600-2_15","title":"Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids","abstract":null,"journal":"Methods in Molecular Biology","year":2018,"id":590346,"datarank":0.3664284135859516,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.09766449320174328,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.09766449320174328,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1510461,"name":"Nina Bertaux-Skeirik","orcid":null,"position":1,"is_corresponding":false},{"id":279063,"name":"Jayati Chakrabarti","orcid":"0000-0003-2265-4657","position":2,"is_corresponding":false},{"id":279062,"name":"Loryn Holokai","orcid":"0000-0002-3611-9535","position":3,"is_corresponding":false},{"id":279071,"name":"Yana Zavros","orcid":"0000-0003-2384-3775","position":4,"is_corresponding":false},{"id":1371900,"name":"Emma Teal","orcid":"0000-0002-5552-4544","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from Organoids","abstract":"Organoid cultures generated from gastrointestinal tissues have been an invaluable advancement for in vitro studies of physiological function and disease. Here we present a comprehensive protocol for the establishment and culture of human- and mouse-derived 3-dimensional gastric organoids transferred to 2-dimensional gastric epithelial cell monolayers. We introduce two methods that include the establishment of monolayers from: (1) intact organoids, and (2) single cells dissociated from intact organoids.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"29959711","pmcid":null,"openalex_id":"https://openalex.org/W2811397904","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"U19 AI116491","title":null}],"total_grants":1,"fwci":2.3377,"citation_percentile":0.91025113,"influential_citations":0,"citation_trend":[{"year":2018,"count":1},{"year":2020,"count":2},{"year":2021,"count":1},{"year":2022,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-4939-8600-2_15","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-4939-8600-2_15","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/29959711","host_type":"repository"}],"fields_of_study":["Cancer Cells and Metastasis","Liver physiology and pathology","Digestive system and related health","Animals","Epithelial Cells","Gastric Mucosa","Humans","Mice","Organoids","Primary Cell Culture"],"mesh_terms":["Animals","Epithelial Cells","Gastric Mucosa","Humans","Organoids","Mice","Primary Cell Culture"],"keywords":["Organoid","Cell biology","In vitro","Cell","Monolayer","Biology","Cell culture","Chemistry","Biochemistry","Regeneration","repair","Stem Cells","Scratch Wound Assay","Gastric Organoids"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-24T18:15:58.322844Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}