{"doi":"10.1007/978-1-4939-7128-2_14","title":"CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish","abstract":null,"journal":"Methods in Molecular Biology","year":2017,"id":623835,"datarank":0.25480476232240024,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.04686060815441662,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.04686060815441662,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":3,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1612418,"name":"Atsuo Kawahara","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish","abstract":"Genome editing technologies such as ZFN, TALEN, and CRISPR/Cas9 efficiently induce DNA double-stranded breaks (DSBs) at a targeted genomic locus, often resulting in a frameshift-mediated target gene disruption. It remains difficult to perform targeted integration of exogenous genes by genome editing technologies. DSBs can be restored through DNA repair mechanisms, such as non-homologous end joining (NHEJ), microhomology-mediated end joining (MMEJ), and homologous recombination (HR). It is well known that HR facilitates homology-dependent integration of donor DNA template into a targeted locus. Recently, both NHEJ-mediated and MMEJ-mediated targeted integrations of exogenous genes have been developed in zebrafish. This chapter summarizes the application of CRISPR/Cas9-mediated knock-in technology in zebrafish.","is_dataset_classified":null,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28643258","pmcid":null,"openalex_id":"https://openalex.org/W2646003346","authors":[],"funders":[],"total_grants":0,"fwci":0.3955,"citation_percentile":0.59398568,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2023,"count":2}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-4939-7128-2_14","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-4939-7128-2_14","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28643258","host_type":"repository"}],"fields_of_study":["CRISPR and Genetic Engineering","Pluripotent Stem Cells Research","Zebrafish Biomedical Research Applications","Medicine","Biology"],"mesh_terms":["Gene Editing","Animals","Recombination, Genetic","Zebrafish","Frameshift Mutation","Genes, Reporter","Gene Knock-In Techniques","CRISPR-Cas Systems"],"keywords":["CRISPR","Genome editing","Cas9","Biology","Transcription activator-like effector nuclease","Zinc finger nuclease","Zebrafish","Homology directed repair","Gene targeting","Homologous recombination","Genetics","Genome engineering","Gene","Non-homologous end joining","Frameshift mutation","Genome","Locus (genetics)","DNA","Computational biology","Gene knockin","DNA repair","Mutation","DNA mismatch repair","Knockout","HR","Nhej","Crispr/cas9","Mmej","Knockin"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T01:34:15.539993Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}