{"doi":"10.1007/978-1-4939-6396-6_16","title":"In Silico Expression Analysis","abstract":null,"journal":"Methods in Molecular Biology","year":2016,"id":651820,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1700128,"name":"Reinhard Hehl","orcid":null,"position":1,"is_corresponding":false},{"id":1700129,"name":"Lorenz Bülow","orcid":null,"position":2,"is_corresponding":false},{"id":1700127,"name":"Julio Bolívar","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"In Silico Expression Analysis","abstract":"Information on the specificity of cis-sequences enables the design of functional synthetic plant promoters that are responsive to specific stresses. Potential cis-sequences may be experimentally tested, however, correlation of genomic sequence with gene expression data enables an in silico expression analysis approach to bioinformatically assess the stress specificity of candidate cis-sequences prior to experimental verification. The present chapter demonstrates an example for the in silico validation of a potential cis-regulatory sequence responsive to cold stress. The described online tool can be applied for the bioinformatic assessment of cis-sequences responsive to most abiotic and biotic stresses of plants. Furthermore, a method is presented based on a reverted in silico expression analysis approach that predicts highly specific potentially functional cis-regulatory elements for a given stress.","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"27557772","pmcid":null,"openalex_id":"https://openalex.org/W2513589746","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.09387262,"influential_citations":0,"citation_trend":[{"year":2015,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-4939-6396-6_16","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-4939-6396-6_16","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/27557772","host_type":"repository"}],"fields_of_study":["Plant Molecular Biology Research","Plant Stress Responses and Tolerance","Plant-Microbe Interactions and Immunity","Cold-Shock Response","Computational Biology","Gene Expression Regulation, Plant","Plants","Promoter Regions, Genetic","Regulatory Sequences, Nucleic Acid","Transcription, Genetic"],"mesh_terms":["Plants","Promoter Regions, Genetic","Regulatory Sequences, Nucleic Acid","Transcription, Genetic","Gene Expression Regulation, Plant","Computational Biology","Cold-Shock Response"],"keywords":["In silico","Computational biology","Biology","Sequence (biology)","Abiotic stress","Gene","Genetics","Bioinformatics","Cis-elements","Expression analysis","Cis-regulatory Sequences","In Silico Validation","Pathoplant","Promoter Sequences"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T10:55:08.474373Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}