{"doi":"10.1007/978-1-0716-3810-1_12","title":"Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders","abstract":null,"journal":"Methods in Molecular Biology","year":2024,"id":662063,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1021572,"name":"Jonas Cerneckis","orcid":"0000-0001-5778-2996","position":1,"is_corresponding":false},{"id":274829,"name":"Yanhong Shi","orcid":"0000-0002-3938-5839","position":2,"is_corresponding":false},{"id":443728,"name":"Cheng Wang","orcid":"0000-0003-0326-2674","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders","abstract":"Human-induced pluripotent stem cell (hiPSC) technology has enabled comprehensive human cell-based disease modeling in vitro. Due to limited accessibility of primary human neurons as well as species-specific divergence between human and rodent brain tissues, hiPSC-derived neurons have become a popular tool for studying neuronal biology in a dish. Here, we provide methods for transcription factor-driven directed differentiation of neurons from hiPSCs via a neural progenitor cell (NPC) intermediate. Doxycycline-inducible expression of neuron fate-determining transcription factors neurogenin 2 (NGN2) and achaete-scute homolog 1 (ASCL1) enables rapid and controllable differentiation of human neurons for disease modeling applications. The provided method is also designed to improve the reproducibility of human neuron differentiation by reducing the batch-to-batch variation of NPC differentiation and lentiviral transduction.","is_dataset_classified":null,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38630226","pmcid":null,"openalex_id":"https://openalex.org/W4394873230","authors":[],"funders":[],"total_grants":0,"fwci":7.3252,"citation_percentile":0.97131964,"influential_citations":0,"citation_trend":[{"year":2024,"count":2},{"year":2025,"count":2},{"year":2026,"count":2}],"oa_status":"closed","license":"https://www.springernature.com/gp/researchers/text-and-data-mining","oa_locations":[{"url":"https://link.springer.com/content/pdf/10.1007/978-1-0716-3810-1_12","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-0716-3810-1_12","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38630226","host_type":"repository"}],"fields_of_study":["Pluripotent Stem Cells Research","CRISPR and Genetic Engineering","3D Printing in Biomedical Research","Humans","Induced Pluripotent Stem Cells","Reproducibility of Results","Neurons","Cell Differentiation","Nervous System Diseases","Transcription Factors"],"mesh_terms":["Cell Differentiation","Humans","Nervous System Diseases","Neurons","Transcription Factors","Reproducibility of Results","Induced Pluripotent Stem Cells"],"keywords":["Induced pluripotent stem cell","Biology","Cellular differentiation","Transcription factor","Neural stem cell","Neuroscience","Neuron","Directed differentiation","Progenitor cell","Cell","Cell biology","Embryonic stem cell","Stem cell","Genetics","Gene","Neurons","Alzheimer’s disease","Stem Cells","Neurological Disorders","Disease Modeling","Ipscs"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life below water"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T12:22:12.430600Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}