{"doi":"10.1007/978-1-0716-3191-1_8","title":"Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interacting RNA","abstract":null,"journal":"Methods in Molecular Biology","year":2023,"id":597746,"datarank":0.24141568686511508,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"self_citation_contribution":0.24141568686511508,"citation_network_contribution":0.0,"self_endowment_contribution":0.24141568686511508,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1531429,"name":"Rami Khosravi","orcid":null,"position":1,"is_corresponding":false},{"id":1531430,"name":"Maayan Salton","orcid":null,"position":2,"is_corresponding":false},{"id":1531428,"name":"Mai Baker","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interacting RNA","abstract":"Proteins with either RNA or DNA-binding motifs were shown to bind RNA. Immunoprecipitation of such proteins using antibodies and identification of the RNA-binding molecules is called RNA immunoprecipitation (RIP). The RNA precipitated with the studied protein can be detected by real-time polymerase chain reaction (PCR), microarray or sequencing. Here, we detail a method for native immunoprecipitation, without cross-linking, to isolate protein-RNA complexes followed by subsequent extraction and quantification of the co-purified RNA.","is_dataset_classified":null,"base_score":1.6094379124341003,"endowment":1.6094379124341003,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"37166660","pmcid":null,"openalex_id":"https://openalex.org/W4376131550","authors":[],"funders":[],"total_grants":0,"fwci":2.788,"citation_percentile":0.90522546,"influential_citations":0,"citation_trend":[{"year":2024,"count":1},{"year":2025,"count":1},{"year":2026,"count":2}],"oa_status":"closed","license":"https://www.springernature.com/gp/researchers/text-and-data-mining","oa_locations":[{"url":"https://link.springer.com/content/pdf/10.1007/978-1-0716-3191-1_8","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-0716-3191-1_8","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/37166660","host_type":"repository"}],"fields_of_study":["RNA Research and Splicing","RNA and protein synthesis mechanisms","RNA modifications and cancer","RNA","RNA-Binding Proteins","Immunoprecipitation"],"mesh_terms":["RNA","RNA-Binding Proteins","Immunoprecipitation"],"keywords":["Immunoprecipitation","RNA","Molecular biology","RNA extraction","RNA-binding protein","Chemistry","Biology","Biochemistry","Gene","Antibody","Real-Time PCR","Native Rna Immunoprecipitation"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Zero hunger"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T14:07:37.468671Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}