{"doi":"10.1007/978-1-0716-2273-5_4","title":"Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Fusarium sp. as a Heterologous Host","abstract":null,"journal":"Methods in Molecular Biology","year":2022,"id":658077,"datarank":0.18806028673362102,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.023268443433404562,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.023268443433404562,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1717880,"name":"Jens Laurids Sørensen","orcid":null,"position":1,"is_corresponding":false},{"id":1717879,"name":"Mikkel Rank Nielsen","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Investigating Fungal Biosynthetic Pathways Using Heterologous Gene Expression: Fusarium sp. as a Heterologous Host","abstract":"Heterologous expression of uncharacterized biosynthetic gene clusters is a popular strategy for exploring the chemical potential of filamentous fungi. Here, we describe the process of PCR-amplifying fungal gene clusters and re-assembling them in a cloning vector via target-associated recombination in Saccharomyces cerevisiae . The gene cluster-carrying construct is validated and used to transform protoplasts of Fusarium graminearum , a well-studied host that is able to express the gene cluster. Chemical analysis of transformants expressing biosynthetic genes can lead to the detection and isolation of novel compounds, such as polyketides.","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35524045","pmcid":null,"openalex_id":"https://openalex.org/W4285121642","authors":[],"funders":[{"funder_name":"Novo Nordisk Fonden","grant_id":"NNF18OC0034952","title":null}],"total_grants":1,"fwci":1.7753,"citation_percentile":0.85407725,"influential_citations":0,"citation_trend":[{"year":2022,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":"cc-by","oa_locations":[{"url":"https://link.springer.com/content/pdf/bfm:978-1-0716-2273-5/1","host_type":"repository"},{"url":"https://link.springer.com/content/pdf/bfm:978-1-0716-2273-5/1","host_type":"repository"},{"url":"https://link.springer.com/content/pdf/10.1007/978-1-0716-2273-5_4","host_type":"publisher"},{"url":"https://link.springer.com/book/10.1007/978-1-0716-2273-5","host_type":"repository"},{"url":"https://doi.org/10.1007/978-1-0716-2273-5_4","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35524045","host_type":"repository"},{"url":"http://www.scopus.com/inward/record.url?scp=85130000441&partnerID=8YFLogxK","host_type":"repository"},{"url":"https://link.springer.com/content/pdf/bfm:978-1-0716-2273-5/1?pdf=chapter%20toc","host_type":"Unpaywall"}],"fields_of_study":["Microbial Natural Products and Biosynthesis","Plant Pathogens and Fungal Diseases","Fungal Biology and Applications","Biosynthetic Pathways","Fusarium","Gene Expression","Multigene Family","Saccharomyces cerevisiae"],"mesh_terms":["Fusarium","Multigene Family","Saccharomyces cerevisiae","Gene Expression","Biosynthetic Pathways"],"keywords":["Heterologous expression","Heterologous","Gene","Gene cluster","Biology","Fusarium","Host (biology)","Gene expression","Computational biology","Genetics","Recombinant DNA","Fungi","Protoplast","Homologous Recombination","Tar","Chromosomal Integration","Protoplast-mediated Transformation"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T03:14:49.059861Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}