{"doi":"10.1007/978-1-0716-0989-7_4","title":"Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc","abstract":null,"journal":"Methods in Molecular Biology","year":2021,"id":688884,"datarank":0.26876392038420827,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.0,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1799699,"name":"Andy Cremers","orcid":null,"position":1,"is_corresponding":false},{"id":1799701,"name":"Donatus A. M. Surtel","orcid":null,"position":2,"is_corresponding":false},{"id":1799702,"name":"Willem Voncken","orcid":null,"position":3,"is_corresponding":false},{"id":1799704,"name":"Tim J. M. Welting","orcid":null,"position":4,"is_corresponding":false},{"id":1799698,"name":"Guus G. H. van den Akker","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc","abstract":"Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.","is_dataset_classified":null,"base_score":1.791759469228055,"endowment":1.791759469228055,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32979197","pmcid":null,"openalex_id":"https://openalex.org/W3089193097","authors":[],"funders":[],"total_grants":0,"fwci":10.5938,"citation_percentile":0.98036606,"influential_citations":0,"citation_trend":[{"year":2021,"count":2},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2025,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-0716-0989-7_4","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-0716-0989-7_4","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/32979197","host_type":"repository"},{"url":"https://cris.maastrichtuniversity.nl/en/publications/7c1f5418-2cf7-4ac0-8bdc-e8e6b75ef0a7","host_type":"repository"}],"fields_of_study":["Spine and Intervertebral Disc Pathology","Orthopaedic implants and arthroplasty","Cervical and Thoracic Myelopathy","Animals","Annulus Fibrosus","Cattle","Cell Separation","Humans","Intervertebral Disc","Nucleus Pulposus","Tissue Engineering"],"mesh_terms":["Nucleus Pulposus","Annulus Fibrosus","Animals","Cattle","Cell Separation","Humans","Intervertebral Disc","Tissue Engineering"],"keywords":["Intervertebral disc","Annulus (botany)","Nucleus","Intervertebral disk","Anatomy","Pathology","Medicine","Biology","Cell biology","Lumbar","Botany","collagenase","cell isolation","Nucleus Pulposus","Cell characterization","annulus fibrosus"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-19T20:06:10.754434Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}