{"doi":"10.1007/978-1-0716-0696-4_3","title":"Mast Cell Purification Protocols","abstract":null,"journal":"Methods in Molecular Biology","year":2020,"id":596388,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1527440,"name":"Elizabeth P. Seward","orcid":null,"position":1,"is_corresponding":false},{"id":1527441,"name":"Peter T. Peachell","orcid":null,"position":2,"is_corresponding":false},{"id":1527439,"name":"Jasmine Farrington","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Mast Cell Purification Protocols","abstract":"Studying a tissue-specific mast cell can be of particular benefit given the heterogeneity that is known to exist among mast cells isolated or developed from different sources. Methods for isolating mast cells from a variety of tissues have been in existence for a number of years although, over time, these methodologies have been refined. We have had considerable experience studying mast cells isolated from human lung tissue. It is for this reason that, in this chapter, we provide detailed methods for the isolation and purification of human lung mast cells. However, it should be noted that the methods that are described in this chapter are generally applicable to the isolation of mast cells from different tissues, and this will also be discussed.","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32766964","pmcid":null,"openalex_id":"https://openalex.org/W4247877958","authors":[],"funders":[],"total_grants":0,"fwci":0.3482,"citation_percentile":0.73825503,"influential_citations":0,"citation_trend":[{"year":2021,"count":1}],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"https://link.springer.com/content/pdf/10.1007/978-1-0716-0696-4_3","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-0716-0696-4_3","host_type":"book series"}],"fields_of_study":["Mast cells and histamine","Asthma and respiratory diseases","Eosinophilic Disorders and Syndromes","Cell Separation","Cells, Cultured","Humans","Lung","Mast Cells","Primary Cell Culture","Proto-Oncogene Proteins c-kit"],"mesh_terms":["Lung","Cells, Cultured","Mast Cells","Humans","Cell Separation","Proto-Oncogene Proteins c-kit","Primary Cell Culture"],"keywords":["Mast (botany)","Mast cell","Biology","Human lung","Immunology","Cell biology","Isolation (microbiology)","Cell culture","Bioinformatics","Genetics","Mast cells","CD117","Dynabeads®"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T10:06:48.357549Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}