{"doi":"10.1007/978-1-0716-0346-8_4","title":"Plasmid-Based Reverse Genetics of Influenza A Virus","abstract":null,"journal":"Methods in Molecular Biology","year":2020,"id":613843,"datarank":0.4636563680037475,"base_score":3.091042453358316,"endowment":3.091042453358316,"self_citation_contribution":0.4636563680037475,"citation_network_contribution":0.0,"self_endowment_contribution":0.4636563680037475,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":21,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":655212,"name":"Brittany Seibert","orcid":"0000-0002-5721-5382","position":1,"is_corresponding":false},{"id":1581564,"name":"Lucas Ferreri","orcid":null,"position":2,"is_corresponding":false},{"id":1581566,"name":"Chang-Won Lee","orcid":null,"position":3,"is_corresponding":false},{"id":1581567,"name":"Daniela Rajao","orcid":null,"position":4,"is_corresponding":false},{"id":1581562,"name":"Daniel R. Perez","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Plasmid-Based Reverse Genetics of Influenza A Virus","abstract":"Reverse genetics is the process of generating an RNA virus from a cDNA copy. Reverse genetics systems have truly transformed our ability to manipulate and study negative-strand RNA viruses. Plasmid-based reverse genetics approaches for influenza viruses provide a better understanding of virulence, transmission, mechanisms of antiviral resistance, and the development of alternative vaccines and vaccination strategies. Studying the molecular changes that allow influenza A viruses (IAVs) to transmit among animal species is important to better understand their animal health and public health risks. In this chapter, the cloning of cDNA copies of IAV's RNA segments into a reverse genetics plasmid vector, the experimental procedures for studying viral polymerase activity, and the successful generation of recombinant IAVs are described.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32170679","pmcid":null,"openalex_id":null,"authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"HHSN272201400008C","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R21 AI146448","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"http://www.springer.com/tdm","oa_locations":[{"url":"http://link.springer.com/content/pdf/10.1007/978-1-0716-0346-8_4","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":["Animals","Dogs","Humans","Influenza A virus","DNA, Complementary","DNA, Viral","RNA, Viral","Cloning, Molecular","Transfection","Polymerase Chain Reaction","Plasmids","HEK293 Cells","Reverse Genetics","Madin Darby Canine Kidney Cells"],"keywords":["Secreted alkaline phosphatase","Influenza A Virus","Virus Rescue","Plasmid-based Reverse Genetics","Bidirectional Plasmids"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T09:23:51.301433Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}