{"doi":"10.1007/978-1-0716-0030-6_7","title":"Lipidomics by HILIC-Ion Mobility-Mass Spectrometry","abstract":null,"journal":"Methods in Molecular Biology","year":2020,"id":625138,"datarank":0.5416376868966337,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"self_citation_contribution":0.5416376868966337,"citation_network_contribution":0.0,"self_endowment_contribution":0.5416376868966337,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":36,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":334291,"name":"Kelly M. Hines","orcid":"0000-0001-9125-5268","position":1,"is_corresponding":false},{"id":334296,"name":"Libin Xu","orcid":"0000-0003-1021-5200","position":2,"is_corresponding":false},{"id":17247,"name":"Amy Li","orcid":"0000-0001-5413-3771","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Lipidomics by HILIC-Ion Mobility-Mass Spectrometry","abstract":"Lipidomics is a rapidly growing field that enables the characterization of the entire lipidome in cells, tissues, or an organism. Changes in lipid metabolism and homeostasis caused by different disease states or drug treatments can be probed by lipidomics experiments, which can aid our understanding of normal physiology and disease pathology at the molecular level. While current technologies using liquid chromatography coupled with high-resolution mass spectrometry have greatly increased coverage of the lipidome, there are still limitations in resolving the large number of lipid species with similar masses in a narrow mass window. We recently reported that two orthogonal separation techniques, hydrophilic interaction liquid chromatography (HILIC) and ion mobility (IM), enhance the resolution of lipid species based on headgroup polarity and gas-phase size and shape, respectively, of various classes of glycerolipids, glycolipids, phospholipids, and sphingolipids. Here we describe the application of our HILIC-IM-MS lipidomics protocol to the analysis of lipid extracts derived from either tissues or cells, to identify significant changes in the lipidome in response to an internal or external stimulus, such as exposure to environmental chemicals.","is_dataset_classified":null,"base_score":3.6109179126442243,"endowment":3.6109179126442243,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"31729657","pmcid":"PMC7255642","openalex_id":"https://openalex.org/W2986402841","authors":[],"funders":[{"funder_name":"NICHD NIH HHS","grant_id":"R01 HD092659","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"T32 GM007750","title":null},{"funder_name":"NICHD NIH HHS","grant_id":"R00 HD073270","title":null},{"funder_name":"National Institutes of Health","grant_id":"5T32GM007750-19","title":"DRUG ACTION, METABOLISM AND KINETICS"},{"funder_name":"National Institutes of Health","grant_id":"1R01HD092659-01","title":"Oxysterols in SLOS Neurodevelopment: Pathological Role and Therapy"},{"funder_name":"National Institutes of Health","grant_id":"5R00HD073270-05","title":"7-Dehydrocholesterol-derived oxysterols in SLOS:  role and therapy"}],"total_grants":6,"fwci":3.5302,"citation_percentile":0.94274538,"influential_citations":0,"citation_trend":[{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":5},{"year":2022,"count":4},{"year":2023,"count":12},{"year":2024,"count":6},{"year":2025,"count":7}],"oa_status":"green","license":"Springer TDM","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7255642","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7255642","host_type":"repository"},{"url":"http://link.springer.com/content/pdf/10.1007/978-1-0716-0030-6_7","host_type":"publisher"},{"url":"https://doi.org/10.1007/978-1-0716-0030-6_7","host_type":"book series"},{"url":"https://pubmed.ncbi.nlm.nih.gov/31729657","host_type":"repository"},{"url":"https://europepmc.org/articles/pmc7255642?pdf=render","host_type":""},{"url":"https://dx.doi.org/10.1007/978-1-0716-0030-6_7","host_type":""}],"fields_of_study":["Metabolomics and Mass Spectrometry Studies","Mass Spectrometry Techniques and Applications","Analytical Chemistry and Chromatography","0301 basic medicine","0303 health sciences","03 medical and health sciences","Chromatography, Liquid","Ion Mobility Spectrometry","Lipidomics","Mass Spectrometry"],"mesh_terms":["Ion Mobility Spectrometry","Lipidomics","Chromatography, Liquid","Mass Spectrometry"],"keywords":["Lipidome","Lipidomics","Hydrophilic interaction chromatography","Chemistry","Mass spectrometry","Ion-mobility spectrometry","Chromatography","Metabolomics","Biochemistry","High-performance liquid chromatography","ion mobility","collision cross section","Hydrophilic Interaction Liquid Chromatography","Ion Mobility Spectrometry","Chromatography, Liquid"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T05:53:33.795851Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}