{"doi":"10.1006/taap.1998.8595","title":"Characterization of the Dose–Response of CYP1B1, CYP1A1, and CYP1A2 in the Liver of Female Sprague–Dawley Rats Following Chronic Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin","abstract":null,"journal":"Toxicology and Applied Pharmacology","year":1999,"id":642210,"datarank":0.6798899239729885,"base_score":4.532599493153256,"endowment":4.532599493153256,"self_citation_contribution":0.6798899239729885,"citation_network_contribution":0.0,"self_endowment_contribution":0.6798899239729885,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":92,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":218795,"name":"Christopher J. Portier","orcid":null,"position":1,"is_corresponding":false},{"id":1670223,"name":"Sigurd F. Lax","orcid":null,"position":2,"is_corresponding":false},{"id":1670224,"name":"Frances G. Crofts","orcid":null,"position":3,"is_corresponding":false},{"id":782936,"name":"Ying Li","orcid":"0000-0002-8029-0399","position":4,"is_corresponding":false},{"id":1670225,"name":"George W. Lucier","orcid":null,"position":5,"is_corresponding":false},{"id":454965,"name":"Thomas R. Sutter","orcid":"0000-0002-6088-6320","position":6,"is_corresponding":false},{"id":542868,"name":"Nigel J. Walker","orcid":"0000-0002-9111-6855","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Characterization of the Dose–Response of CYP1B1, CYP1A1, and CYP1A2 in the Liver of Female Sprague–Dawley Rats Following Chronic Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin","abstract":"One of the current knowledge gaps in the evaluation of risk for human exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is the relationship between gene expression induced by TCDDmore complex biological responses such as altered growth, differentiation, and neoplasia. This study investigates the dose-dependent expression of CYP1A1, CYP1A2,CYP1B1 in the livers of female Sprague-Dawley rats chronically exposed to TCDD. Animals were treated biweekly for 30 weeks with daily averaged doses of 0 to 125 ng TCDD/kg/day. Immunoblot analysis showed that protein levels for CYP1B1, CYP1A1, CYP1A2 exhibited a dose-dependent induction by TCDD. However, CYP1A1 and CYP1A2 protein levels were approximately 100-fold higher than CYP1B1, which could not be detected by either immunoblot analysis or immunohistochemistry in the livers of rats treated with TCDD for 30 weeks at a dose-equivalent less than 35.7 ng/kg/day. In control animals, CYP1A1CYP1A2 RNA levels, measured by quantitative RT-PCR, were 1100-15,000-fold higher than that of CYP1B1, respectively. TCDD induced CYP1B1 RNA levels at all doses, although absolute TCDD-induced levels of CYP1A1CYP1A2 at the highest dose (125 ng/kg/day) were more than 40-fold higher than that of CYP1B1. While the liver concentration of TCDD required for half-maximal induction of CYP1A1, CYP1A2,CYP1B1 RNA levels was similar, the shaping parameter (Hill coefficient) of the dose-response curve for CYP1B1 was significantly higher than that for CYP1A1 or CYP1A2. The low level of TCDD-induced CYP1B1 expression in the liver relative to that of the CYP1A1CYP1A2 suggest that, if CYP1B1 is involved in TCDD-induced hepatocarcinogenesis, its endogenous function is likely to be uniquenot overlapping with that of CYP1A1 or CYP1A2.","is_dataset_classified":null,"base_score":4.532599493153256,"endowment":4.532599493153256,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9931287","pmcid":null,"openalex_id":"https://openalex.org/W2051399550","authors":[],"funders":[{"funder_name":"NIEHS NIH HHS","grant_id":"ES08148","title":null},{"funder_name":"NIEHS NIH HHS","grant_id":"ES03819","title":null},{"funder_name":"NIEHS NIH HHS","grant_id":"ES07141","title":null}],"total_grants":3,"fwci":8.5546,"citation_percentile":0.98769079,"influential_citations":0,"citation_trend":[{"year":2012,"count":4},{"year":2013,"count":1},{"year":2014,"count":2},{"year":2015,"count":1},{"year":2016,"count":1},{"year":2018,"count":2},{"year":2022,"count":1},{"year":2024,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0041008X98985957?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0041008X98985957?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/taap.1998.8595","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9931287","host_type":"repository"}],"fields_of_study":["Toxic Organic Pollutants Impact","Carcinogens and Genotoxicity Assessment","Pharmacogenetics and Drug Metabolism"],"mesh_terms":["Polychlorinated Dibenzodioxins","Animals","Aryl Hydrocarbon Hydroxylases","Cytochrome P-450 Enzyme System","Dose-Response Relationship, Drug","Enzyme Induction","Female","Gene Expression Regulation","Liver","Microsomes, Liver","RNA, Messenger","Time","Immunoblotting","Rats, Sprague-Dawley","Cytochrome P-450 CYP1A1","Cytochrome P-450 CYP1A2","Reverse Transcriptase Polymerase Chain Reaction","Rats","Cytochrome P-450 CYP1B1"],"keywords":["CYP1A2","CYP1B1","Internal medicine","Endocrinology","Cytochrome P450","Dose–response relationship","Chemistry","Messenger RNA","Toxicity","Biology","Metabolism","Medicine","Biochemistry","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-07T21:56:11.996583Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}