{"doi":"10.1006/plas.1998.1357","title":"A Versatile Prokaryotic Cloning Vector with Six Dual Restriction Enzyme Sites in the Polylinker Facilitates Efficient Subcloning into Vectors with Unique Cloning Sites","abstract":null,"journal":"Plasmid","year":1998,"id":661552,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1727059,"name":"Antoinette C. Chillemi","orcid":null,"position":1,"is_corresponding":false},{"id":446113,"name":"Joyce D. Fingeroth","orcid":"0000-0003-1620-1731","position":2,"is_corresponding":false},{"id":1727056,"name":"David R. Sage","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A Versatile Prokaryotic Cloning Vector with Six Dual Restriction Enzyme Sites in the Polylinker Facilitates Efficient Subcloning into Vectors with Unique Cloning Sites","abstract":"In large and complex vectors a single restriction enzyme recognition site may be available for introduction of additional DNA requiring the development of linker fragments to create compatible insertion sites. This technology can be time consuming and costly. We describe the construction of a simple phagemid, pSFI, with a polylinker that contains six pairs of dual, rare-cutting, restriction enzyme recognition sites (NotI, SpeI, EcoRV, PstI, SacII, EagI) with multiple unique sites between each pair. This has permitted rapid subcloning of DNA with creation of single flanking restriction enzyme sites. pSFI was used to expedite transfer of viral genes to a LacZ-inducible expression vector and to an adenovirus expression cassette for production of replication-defective virus. The use of this phagemid has facilitated complex vector manipulations and is a valuable adjunct to the family of multifunctional cloning vectors.","is_dataset_classified":null,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9735318","pmcid":null,"openalex_id":"https://openalex.org/W1968083434","authors":[],"funders":[{"funder_name":"NIDCR NIH HHS","grant_id":"R01 DE12186","title":null}],"total_grants":1,"fwci":0.1958,"citation_percentile":0.54236608,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0147619X98913579?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0147619X98913579?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/plas.1998.1357","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9735318","host_type":"repository"}],"fields_of_study":["Virus-based gene therapy research","Bacteriophages and microbial interactions","CRISPR and Genetic Engineering"],"mesh_terms":["Bacteriophages","Base Sequence","Binding Sites","DNA Restriction Enzymes","DNA, Viral","Genetic Vectors","Molecular Sequence Data","Prokaryotic Cells"],"keywords":["Subcloning","Multiple cloning site","Restriction enzyme","Cloning vector","Biology","Restriction site","Cloning (programming)","Genetics","Shuttle vector","Phagemid","Cosmid","EcoRV","Vector (molecular biology)","Computational biology","Plasmid","EcoRI","DNA","Gene","Recombinant DNA","Bacteriophage","Computer science","Escherichia coli"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T11:12:52.407411Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}