{"doi":"10.1006/mthe.2002.0700","title":"Noninvasive Imaging of Cationic Lipid-Mediated Delivery of Optical and PET Reporter Genes in Living Mice","abstract":null,"journal":"Molecular Therapy","year":2002,"id":629332,"datarank":0.6010999777848708,"base_score":4.007333185232471,"endowment":4.007333185232471,"self_citation_contribution":0.6010999777848708,"citation_network_contribution":0.0,"self_endowment_contribution":0.6010999777848708,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":54,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1428305,"name":"Manijeh Berenji","orcid":"0000-0001-7255-8837","position":1,"is_corresponding":false},{"id":1629774,"name":"Nancy S. Templeton","orcid":null,"position":2,"is_corresponding":false},{"id":232231,"name":"Sanjiv S. Gambhir","orcid":"0000-0002-2711-7554","position":3,"is_corresponding":false},{"id":1629771,"name":"Meera Iyer","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Noninvasive Imaging of Cationic Lipid-Mediated Delivery of Optical and PET Reporter Genes in Living Mice","abstract":"Gene therapy involves the safe and effective delivery of one or more genes of interest to target cells in vivo. The advantages of using nonviral delivery systems include ease of preparation, low toxicity, and weak immunogenicity. Nonviral delivery methods, when combined with a noninvasive, clinically applicable imaging assay, will greatly aid in the optimization of gene therapy approaches for cancer. We demonstrate cationic lipid-mediated noninvasive monitoring of reporter gene expression of firefly (Photinus pyralis) luciferase (fl) and a mutant herpes simplex virus type I thymidine kinase (HSV1-sr39tk, tk) in living mice using a cooled charge coupled device (CCD) camera and positron emission tomography (PET), respectively. We observe a high level of fl and tk reporter gene expression predominantly in the lungs after a single injection of the extruded DOTAP:cholesterol DNA liposome complexes by way of the tail vein, seen to be time- and dose-dependent. We observe a good correlation between the in vivo bioluminescent signal and the ex vivo firefly luciferase enzyme (FL) activity in different organs. We further demonstrate the feasibility of noninvasively imaging both optical and PET reporter gene expression in the same animal using the CCD camera and microPET, respectively.","is_dataset_classified":null,"base_score":4.007333185232471,"endowment":4.007333185232471,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"12385291","pmcid":null,"openalex_id":"https://openalex.org/W2040792816","authors":[],"funders":[{"funder_name":"NCI NIH HHS","grant_id":"R24 CA92865","title":null},{"funder_name":"NCI NIH HHS","grant_id":"R01 CA82214-01","title":null},{"funder_name":"National Institutes of Health","grant_id":"5R24CA092865-03","title":"UCLA Imaging Resource for Mouse Cancer Models"}],"total_grants":3,"fwci":4.8921,"citation_percentile":0.95409057,"influential_citations":0,"citation_trend":[{"year":2014,"count":2},{"year":2016,"count":1},{"year":2018,"count":2},{"year":2021,"count":1},{"year":2025,"count":1}],"oa_status":"hybrid","license":"cc-by-nc-nd","oa_locations":[{"url":"http://www.cell.com/article/S1525001602907008/pdf","host_type":"journal"},{"url":"http://www.cell.com/article/S1525001602907008/pdf","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1525001602907008?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S1525001602907008?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/mthe.2002.0700","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/12385291","host_type":"repository"},{"url":"http://dx.doi.org/10.1006/mthe.2002.0700","host_type":""},{"url":"https://dx.doi.org/10.1006/mthe.2002.0700","host_type":""}],"fields_of_study":["Virus-based gene therapy research","RNA Interference and Gene Delivery","Viral Infectious Diseases and Gene Expression in Insects","0301 basic medicine","0303 health sciences","03 medical and health sciences"],"mesh_terms":["Animals","DNA","Kinetics","Liposomes","Lung","Tomography, Emission-Computed","Genes, Reporter","Gene Transfer Techniques","Gene Dosage","Mice"],"keywords":["Reporter gene","Luciferase","Genetic enhancement","Gene delivery","In vivo","Molecular biology","Thymidine kinase","Preclinical imaging","Cationic liposome","Vectors in gene therapy","Bioluminescence imaging","Herpes simplex virus","Gene expression","Transfection","Biology","Chemistry","Gene","Viral vector","Recombinant DNA","Biochemistry","Virology","Virus","Genetics","Pharmacology","Gene Dosage","Gene Transfer Techniques","DNA","Kinetics","Mice","Genes, Reporter","Drug Discovery","Liposomes","Molecular Medicine","Animals","Lung","Tomography, Emission-Computed"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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