{"doi":"10.1006/jmbi.1996.0522","title":"The Preprotein Translocase of the Inner Mitochondrial Membrane: Evolutionary Conservation of Targeting and Assembly of Tim17","abstract":null,"journal":"Journal of Molecular Biology","year":1996,"id":662302,"datarank":0.6238324625039509,"base_score":4.1588830833596715,"endowment":4.1588830833596715,"self_citation_contribution":0.6238324625039509,"citation_network_contribution":0.0,"self_endowment_contribution":0.6238324625039509,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":63,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1728962,"name":"Joachim Rassow","orcid":null,"position":1,"is_corresponding":false},{"id":1728963,"name":"Nicole Zufall","orcid":null,"position":2,"is_corresponding":false},{"id":1230472,"name":"Nikolaus Pfanner","orcid":"0000-0003-2136-2046","position":3,"is_corresponding":false},{"id":1728964,"name":"Michiel Meijer","orcid":null,"position":4,"is_corresponding":false},{"id":1728965,"name":"Ammy C. Maarse","orcid":null,"position":5,"is_corresponding":false},{"id":1728961,"name":"Ulf Bömer","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The Preprotein Translocase of the Inner Mitochondrial Membrane: Evolutionary Conservation of Targeting and Assembly of Tim17","abstract":"The preprotein translocase of the inner mitochondrial membrane has only been described in Saccharomyces cerevisiae to date. We report that the essential subunit Tim17 is highly conserved in evolution. The targeting and assembly of yeast Tim17 as well as that of human and Drosophila melanogaster Tim17 were characterized with isolated yeast mitochondria. Targeting signals in the mature protein direct the Tim17 precursors to the receptor Tom70 on the mitochondrial surface. In a membrane potential-dependent step the precursors insert into the inner membrane, adopt a characteristic topology and assemble with Tim23. The mechanisms of targeting and assembly were indistinguishable between the Tim17s from distinct organisms, indicating a high evolutionary conservation.","is_dataset_classified":null,"base_score":4.1588830833596715,"endowment":4.1588830833596715,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8893850","pmcid":null,"openalex_id":"https://openalex.org/W2126125209","authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2012,"count":2},{"year":2015,"count":2},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":1},{"year":2020,"count":2},{"year":2021,"count":3},{"year":2023,"count":1},{"year":2024,"count":2}],"oa_status":"closed","license":"https://www.elsevier.com/tdm/userlicense/1.0/","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0022283696905227?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0022283696905227?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/jmbi.1996.0522","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8893850","host_type":"repository"},{"url":"https://dare.uva.nl/personal/pure/en/publications/the-preprotein-translocase-of-the-inner-mitochondrial-membrane-evolutionary-conservation-of-targeting-and-assembly-of-tim17(004684c2-f340-4617-8d75-49ba1d9f9663).html","host_type":"repository"},{"url":"https://handle.uba.uva.nl/personal/pure/en/publications/the-preprotein-translocase-of-the-inner-mitochondrial-membrane-evolutionary-conservation-of-targeting-and-assembly-of-tim17(004684c2-f340-4617-8d75-49ba1d9f9663).html","host_type":"repository"},{"url":"https://freidok.uni-freiburg.de/data/19168","host_type":"repository"}],"fields_of_study":["Mitochondrial Function and Pathology","RNA and protein synthesis mechanisms","Genomics and Phylogenetic Studies","Adenosine Triphosphatases","Amino Acid Sequence","Animals","Bacterial Proteins","Base Sequence","Biological Transport","Carrier Proteins","Conserved Sequence","Drosophila melanogaster","Escherichia coli Proteins","Humans","Intracellular Membranes","Membrane Proteins","Membrane Transport Proteins","Mitochondria","Mitochondrial Membrane Transport Proteins","Mitochondrial Precursor Protein Import Complex Proteins","Molecular Sequence Data","Protein Conformation","Rats","Repressor Proteins","SEC Translocation Channels","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","SecA Proteins"],"mesh_terms":["SEC Translocation Channels","SecA Proteins","Mitochondrial Precursor Protein Import Complex Proteins","Adenosine Triphosphatases","Amino Acid Sequence","Animals","Bacterial Proteins","Base Sequence","Biological Transport","Carrier Proteins","Drosophila melanogaster","Humans","Intracellular Membranes","Membrane Proteins","Mitochondria","Molecular Sequence Data","Protein Conformation","Repressor Proteins","Saccharomyces cerevisiae","Conserved Sequence","Membrane Transport Proteins","Saccharomyces cerevisiae Proteins","Escherichia coli Proteins","Mitochondrial Membrane Transport Proteins","Rats"],"keywords":["Translocase","Translocase of the inner membrane","Translocase of the outer membrane","Inner mitochondrial membrane","Intermembrane space","Inner membrane","Protein targeting","Cell biology","Protein subunit","Biology","Mitochondrial carrier","Mitochondrion","Yeast","ATP–ADP translocase","Conserved sequence","Biochemistry","Membrane protein","Mitochondrial membrane transport protein","Bacterial outer membrane","Membrane","Peptide sequence","Gene"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Life in Land"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-12T13:41:11.370370Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}