{"doi":"10.1006/clin.1998.4545","title":"Human Neutrophils Express the Prostaglandin G/H Synthase 2 Gene When Stimulated with Bacterial Lipopolysaccharide","abstract":null,"journal":"Clinical Immunology and Immunopathology","year":1998,"id":602567,"datarank":0.47670807455219194,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"self_citation_contribution":0.47670807455219194,"citation_network_contribution":0.0,"self_endowment_contribution":0.47670807455219194,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":23,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1545383,"name":"Jon D. Wells","orcid":null,"position":1,"is_corresponding":false},{"id":483155,"name":"Charles E. McCall","orcid":"0000-0002-9082-469X","position":2,"is_corresponding":false},{"id":1545382,"name":"Mary B. Fasano","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Human Neutrophils Express the Prostaglandin G/H Synthase 2 Gene When Stimulated with Bacterial Lipopolysaccharide","abstract":"Human blood neutrophils (PMN) rapidly release arachidonic acid (AA) from cellular phospholipids when stimulated in vitro with a variety of inflammatory agonists. Free AA is then metabolized via 5'-lipoxygenase to produce bioactive mediators such as leukotriene B4 and 5-hydroxyeicosatetraenoate. Arachidonic acid can also be metabolized via the cyclooxygenase or prostaglandin G/H synthase (PGHS) pathway to form prostaglandins and thromboxane. We show here that human blood PMN express the PGHS 2 gene when stimulated with bacterial lipopolysaccharide (LPS). PGHS 2 mRNA increases within 30 min after LPS stimulation and PGHS 2 immunoreactive protein is detectable by 5 h. Although PGHS 1 mRNA is detectable in PMN, no immunoreactive protein is observed in either resting or LPS-stimulated cells. Following stimulation with LPS and expression of PGHS 2, PMN increase secretion of prostaglandin E2. This phenotypic change in PMN could be an important mechanism for regulating inflammation.","is_dataset_classified":null,"base_score":3.1780538303479458,"endowment":3.1780538303479458,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9646841","pmcid":null,"openalex_id":"https://openalex.org/W2016136675","authors":[],"funders":[{"funder_name":"NHLBI NIH HHS","grant_id":"P0-1 HL-50395","title":null},{"funder_name":"NHLBI NIH HHS","grant_id":"HL-29293","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"AI-09169","title":null}],"total_grants":3,"fwci":1.3684,"citation_percentile":0.82217128,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2016,"count":3},{"year":2017,"count":1},{"year":2022,"count":1},{"year":2023,"count":1}],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0090122998945457?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0090122998945457?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/clin.1998.4545","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9646841","host_type":"repository"}],"fields_of_study":["Inflammatory mediators and NSAID effects","Immune Response and Inflammation","Neutrophil, Myeloperoxidase and Oxidative Mechanisms"],"mesh_terms":["Cells, Cultured","Humans","Lipopolysaccharides","Neutrophils","Prostaglandin-Endoperoxide Synthases","RNA, Messenger","Stimulation, Chemical","Dinoprostone","Gene Expression Regulation, Enzymologic"],"keywords":["Arachidonic acid","Lipopolysaccharide","Eicosanoid","Stimulation","Prostaglandin","Cyclooxygenase","Leukotriene","Secretion","Endocrinology","Prostaglandin E2","Internal medicine","Biology","Lipoxygenase","Thromboxane A2","Inflammation","Thromboxane B2","Chemistry","Biochemistry","Enzyme","Immunology","Medicine","Receptor","Platelet"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T19:44:17.844560Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}