{"doi":"10.1006/cimm.1994.1007","title":"Appearance of Extrathymic Early Differentiated CD4-CD8- T Cells with T Cell Receptor γ/δ or α/β after Thymus Grafting to Nude Mice: Influence of Thymus on Extrathymic T Cell Differentiation","abstract":null,"journal":"Cellular Immunology","year":1994,"id":648492,"datarank":0.37204306259500963,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.16409890842702604,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.16409890842702604,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":3,"citers_with_citation_signal":3,"citers_with_endowment":3,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1532359,"name":"Yasunobu Yoshikai","orcid":null,"position":1,"is_corresponding":false},{"id":1690072,"name":"Goro Matsuzaki","orcid":null,"position":2,"is_corresponding":false},{"id":1690074,"name":"Akinori Iwasaki","orcid":null,"position":3,"is_corresponding":false},{"id":1690076,"name":"Hiroyuki Yuuki","orcid":null,"position":4,"is_corresponding":false},{"id":1560863,"name":"Takao Nakamura","orcid":null,"position":5,"is_corresponding":false},{"id":1690077,"name":"Kikuo Nomoto","orcid":null,"position":6,"is_corresponding":false},{"id":1690070,"name":"Hiroyuki Kenai","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Appearance of Extrathymic Early Differentiated CD4-CD8- T Cells with T Cell Receptor γ/δ or α/β after Thymus Grafting to Nude Mice: Influence of Thymus on Extrathymic T Cell Differentiation","abstract":"Fetal thymus grafting into athymic nude mice has been used as an experimental model of T cell development. To understand the early events of T cell development, we have examined the sequence of appearance of T cell subsets in lymph nodes (LN) of BALB/c nu/nu mice after grafting with syngeneic fetal thymus. T cells expressing T cell receptor (TCR) alpha/beta or gamma/delta increased in LN from 1 week after grafting, although no host-derived CD3+ T cells were detected in the grafted thymus and no donor thymus-derived T cells were detected in the LN. The early appearing T cells of both TCR alpha/beta and TCR gamma/delta showed a CD4-CD8- phenotype. V region usage analysis of the early appearing TCR alpha/beta T cells revealed that they contained cells bearing V beta 3 or V beta 11, which are potentially reactive to self-superantigen Mls-2a or Dvb11, respectively, and are deleted in the course of T cell development in the thymus of euthymic BALB/c mice. The early appearing T cells showed neither mixed lymphocyte reaction nor cytotoxic T cell activity against allogeneic cells. In contrast, lymphokine-activated killer cells from early appearing T cells, which contained high percentages of TCR gamma/delta T cells, exhibited higher cytotoxic activity against P815 mastocytoma than those from euthymic mice or untreated nude mice. All these results suggest that the early appearing T cells are developed extrathymically. We propose that the thymus may induce extrathymic T cell development without direct cell-to-cell interaction. It seems likely that the extrathymically developed T cells, especially TCR gamma/delta T cells, induced by the thymus have some role in the defense mechanism in the absence of conventional thymus-derived T cells.","is_dataset_classified":null,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"8287494","pmcid":null,"openalex_id":"https://openalex.org/W2045231844","authors":[],"funders":[],"total_grants":0,"fwci":0.2527,"citation_percentile":0.50058445,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"https://www.elsevier.com/legal/tdmrep-license","oa_locations":[{"url":"https://api.elsevier.com/content/article/PII:S0008874984710070?httpAccept=text/xml","host_type":"publisher"},{"url":"https://api.elsevier.com/content/article/PII:S0008874984710070?httpAccept=text/plain","host_type":"publisher"},{"url":"https://doi.org/10.1006/cimm.1994.1007","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/8287494","host_type":"repository"}],"fields_of_study":["T-cell and B-cell Immunology","Immune Cell Function and Interaction","CAR-T cell therapy research"],"mesh_terms":["Animals","Cell Differentiation","Cytotoxicity, Immunologic","Female","Killer Cells, Natural","Lymph Nodes","Lymphocyte Activation","Mice, Inbred AKR","Mice, Inbred BALB C","Mice, Inbred C57BL","Mice, Nude","Thymus Gland","Killer Cells, Lymphokine-Activated","T-Lymphocyte Subsets","Receptors, Antigen, T-Cell, gamma-delta","Receptors, Antigen, T-Cell, alpha-beta","Mice"],"keywords":["Cytotoxic T cell","Biology","T-cell receptor","CD8","T cell","T lymphocyte","Thymocyte","Molecular biology","CD3","Interleukin 21","Mastocytoma","Immunology","Cell biology","Cancer research","Antigen","Immune system","In vitro","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-10T02:40:24.785129Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}